Astragalus membranaceus promote expression of insulin-like growth factor 1 in rat model of olivo-cerebellar degeneration.
- Author:
Hui-ling WU
1
;
Hui-jun ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Astragalus membranaceus; chemistry; Calbindins; Cerebellum; drug effects; metabolism; Dose-Response Relationship, Drug; Drugs, Chinese Herbal; administration & dosage; isolation & purification; pharmacology; Enzyme-Linked Immunosorbent Assay; Gene Expression; drug effects; Immunohistochemistry; Insulin-Like Growth Factor I; biosynthesis; genetics; Male; Motor Activity; drug effects; Neuroprotective Agents; administration & dosage; isolation & purification; pharmacology; Olivary Nucleus; drug effects; metabolism; Plants, Medicinal; chemistry; Pyridines; RNA, Messenger; biosynthesis; genetics; Random Allocation; Rats; Rats, Sprague-Dawley; Reverse Transcriptase Polymerase Chain Reaction; S100 Calcium Binding Protein G; metabolism; Spinocerebellar Degenerations; blood; chemically induced; metabolism
- From: China Journal of Chinese Materia Medica 2007;32(3):242-245
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect of Astragalus membranaceus (AM) on insulin-like growth factor 1 (IGF-1) expression in a rat model of olivo-cerebellar degeneration and assess the neuroprotective actions of AM meanwhile.
METHODRats model of olivo-cerebellar degeneration was established by using 3-acetylpyridine. The effect of AM on the expression of Calbindin D-28K in inferior olive (IO) neurons by immunohistochemistry, the serum IGF-1 level by Elisa, the IGF-1 mRNA level in the cerebellum by RT-PCR were detected respectively.
RESULTAM effectively improve the serum IGF-1 level, Cerebellar IGF-1 mRNA level and the survival of the 10 neurons in a rat model of olivo-cerebellar degeneration, even at a lower dose (9 g x kg(-1)), and the effect was in a dose-dependent manner.
CONCLUSIONAM could effectively upregulate the IGF-1 expression in the rat model of olivo-cerebellar degeneration, and have neuroprotective effect on IO neurons.
