Osteoblastic early attachment onto the surface of bioadhesive peptide modified pure titanium
10.3760/cma.j.issn.1002-0098.2010.08.005
- VernacularTitle:黏附肽改性纯钛表面成骨细胞的早期黏附能力研究
- Author:
Yi-Fan CHEN
1
;
Yuan-Jin HUANG
;
Guang-Bao SONG
;
Qian-Bing WAN
;
Jian WANG
;
Yong-Lie CHAO
Author Information
1. 南方医科大学附属口腔医院(广东省口腔医院)
- Keywords:
Titanium;
Peptides;
Osteoblasts
- From:
Chinese Journal of Stomatology
2010;45(8):462-465
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the long-term integrity and the biological function of interface between the bioadhesive peptide modified implant surface and peri-implant tissue. Methods A short bioadhesive peptide containing Glyeine-Tyrosine-Arginine-Glycine-Asparticacid-Serine(GYRGDS) sequence was immobilized onto the titanium implant surface by means of sol-gel coating technique and self-assembled monolayers(SAM). The chemical composition and organic functional groups on the titanium surfaces were characterized using XPS (X-ray photoelectron spectroscopy ) and FTIR (Fourier transform infrared spectrometer). The adhesive strength and stability of osteoblasts on various implant surfaces were compared under flow condition. Results The results showed that alkali/hot water aging treatment could apparently improve the content of -OH functional groups of titanium surface. The chemical reactive Ti-O-Ti bonding at the surface of titanium played a vital role in inducing the formation of organosilane SAM. GYRGDS peptide can be covalently grafted onto the surface of titanium by SAM technique. The resistance of freshly adherent osteoblasts to detachment by flow was shear time dependent. When the four groups were compared under the same flow stress condition(2.05 Pa) at three specific time spans(30 min, 1 h, 2 h), the cells retention rates in GYRGDS-grafted groups were 93.0%, 54. 4%, 34. 4% respectively and were much higher than those in non-coated groups. Conclusions It was suggested that GYRGDS might have positive effects on maintaining stability and adherence of cells onto the substrates under flow condition.
