Expression of IL-6 and it's mRNA transcription in the process of rat periodontium remodeling influenced by various bite forces.
- Author:
Lin YUAN
1
;
Yunfeng ZHAO
;
Weidong ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bite Force; Immunohistochemistry; In Situ Hybridization; Interleukin-6; analysis; genetics; Male; Periodontium; physiology; RNA, Messenger; analysis; Rats; Rats, Wistar; Transcription, Genetic
- From: Chinese Journal of Stomatology 2002;37(1):54-57
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the expression of IL-6 in periodontal ligament fibroblasts (PLF) and osteoblasts in order to explain the molecular mechanism of the bite forte on periodontium remodeling.
METHODSA rat model of different bite forces was established to study the expression of IL-6 and IL-6 mRNA in the PLF and osteoblasts with immunohistochemical (IHC) and in situ hybridization (ISH) techniques.
RESULTSIn the group of disuse bite force, the expression of IL-6 and IL-6 mRNA in the PLF and osteoblasts enhanced apparently compared with that in the group of normal bite force. The gray level values enhanced respectively from 6.5886 +/- 1.6 to 38.8381 +/- 9.5 and from 11.8337 +/- 2.8 to 57.2623 +/- 13.6. The loosely arrangement of periodontal fibers and the resorption of alveolar bone were observed in histological study. In the group of compensation bite force, the same result was observed with IHC and ISH, the gray level values enhanced respectively from 6.3119 +/- 1.5 to 45.5456 +/- 11.1 and from 11.7714 +/- 2.8 to 81.9391 +/- 19.5, but the increase of periodontal fibers, the resorption and formation of alveolar bone were observed simultaneity in histological study.
CONCLUSIONSWith the change of bite force, the expression of IL-6 and IL-6 mRNA in PLF and osteoblasts presents a certain regulation. IL-6 may play important roles in the effect of bite force on periodontium remodeling.
