Investigation on long-term survival of transplanted bone marrow mesenchymal stem cells in infarcted myocardium of rats
10.3760/cma.j.issn.0253-3758.2011.02.019
- VernacularTitle:大鼠骨髓间充质干细胞移植在心肌梗死区长期存活的观察
- Author:
Hong-Min LI
1
;
Lin LIU
;
Xiang MEI
;
Xue ZHAO
Author Information
1. 第二军医科大学长征医院
- Keywords:
Myocardial infarction;
Stem cell transplantation
- From:
Chinese Journal of Cardiology
2011;39(2):171-175
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the quantification and distribution of survival transplanted bone marrow mesenchymal stem cells (BMSCs) in infarcted myocardium of rats. Methods BMSCs of male SD rats were isolated, purified and proliferated. Female SD rats were subjected to occlusion of left anterior descending artery, 1.2 × 106 BMSCs were injected into the centre zone of the infarct region 1 h after left anterior descending artery occlusion. Myocardial tissue was harvested randomly at 1,3 and 6 ( n = 18) weeks after transplantation. Transplanted cells were labeled with Hoechst33342 ( n = 5 ) and Brdu ( n = 5 ). Sry suquence of Y chromatosome in male rat was analyzed by real-time PCR in the tissue ( n = 8). Results FACS analysis showed that BMSCs expressed homogenous with CD44 +/ CD34 -. Hoechst33342 or Brdu labeled positive cells were found in localized accumulation and some of them migrated to the margin of the infarct region, and vessel-like structure could be observed in the host hearts. The real-time PCR demonstrated that the survival rates of engrafted BMSCs were 7. 88%, 7. 82% and 8. 73% respectively at 1,3 and 6 weeks after transplantation. Conclusion BMSCs could survive in the host infarcted myocardium for more than one month and the survival rate was around 8% from the first week to the sixth week after transplantation.
