Advance of gene-modified non-human primate models.

Author: Zhen LIU ¹ ; Yijun CAI ¹ ; Qiang SUN ¹
Author Information

1. State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Institute of Neuroscience, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
Publication Type:Journal Article
Keywords: gene-modified; lentivirus; non-human primate; programmable nucleases
From: Chinese Journal of Biotechnology 2017;33(10):1665-1673
CountryChina
Language:Chinese
Abstract: Non-human primates would be particularly valuable in life sciences and biomedical research area. Gene-modified monkeys with gene overexpression or loss of function have been successfully generated with the rapid advance in gene manipulation technology such as lentivirus infection and programmable nucleases (ZFN, TALEN, CRISPR-Cas9). Here we review the recent development on gene-modified monkey generation by lentivirus and programmable nucleases. Then we discuss three concerns, the long time for sexual maturation, the off target and the mosaicism of founders, which limit the wide application of gene-modified non-human-primates. At last, hotspots and future trend for gene-modified non-human-primates generation are proposed.