Advances in CRISPR-Cas-mediated genome editing system in plants.
- Author:
Chun WANG
1
;
Kejian WANG
1
Author Information
- Publication Type:Journal Article
- Keywords: CRISPR-Cas system; crop genetics and breeding; genome editing; plant genomes
- From: Chinese Journal of Biotechnology 2017;33(10):1712-1722
- CountryChina
- Language:Chinese
- Abstract: Targeted genome editing technology is an important tool to study the function of genes and to modify organisms at the genetic level. Recently, CRISPR-Cas (clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins) system has emerged as an efficient tool for specific genome editing in animals and plants. CRISPR-Cas system uses CRISPR-associated endonuclease and a guide RNA to generate double-strand breaks at the target DNA site, subsequently leading to genetic modifications. CRISPR-Cas system has received widespread attention for manipulating the genomes with simple, easy and high specificity. This review summarizes recent advances of diverse applications of the CRISPR-Cas toolkit in plant research and crop breeding, including expanding the range of genome editing, precise editing of a target base, and efficient DNA-free genome editing technology. This review also discusses the potential challenges and application prospect in the future, and provides a useful reference for researchers who are interested in this field.
