Genome-editing: focus on the off-target effects.

Author: Xiubin HE ¹ ; Feng GU ¹
Author Information

1. State Key Laboratory of Ophthalmology and Optometry, School of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou 325000, Zhejiang, China.
Publication Type:Journal Article
Keywords: clustered regulatory interspaced short palindromic repeats (CRISPRs); genome editing; off-target; transcription activator-like effector nucleases (TALENs); zinc-finger nucleases (ZFNs)
From: Chinese Journal of Biotechnology 2017;33(10):1757-1775
CountryChina
Language:Chinese
Abstract: Breakthroughs of genome-editing in recent years have paved the way to develop new therapeutic strategies. These genome-editing tools mainly include Zinc-finger nucleases (ZFNs), Transcription activator-like effector nucleases (TALENs), and clustered regulatory interspaced short palindromic repeat (CRISPR)/Cas-based RNA-guided DNA endonucleases. However, off-target effects are still the major issue in genome editing, and limit the application in gene therapy. Here, we summarized the cause and compared different detection methods of off-targets.