Effect of zoledronic acid on the differentiation and osteoprotegerin production of osteoblasts in rabbit

VernacularTitle:唑来膦酸对兔成骨细胞分化及骨保护素分泌的影响
Author: Jian ZHANG ¹ ; Wen-Yi ZHANG ; Hua SUN
Author Information

1. 天津医科大学口腔医院
Keywords: Osteoblasts; Alkaline phosphatase; Zoledronic acid; Osteoprotegerin
From: Chinese Journal of Stomatology 2010;45(8):502-505
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of zoledronic acid on proliferation and osteogenic differentiation of esteoblasts. Methods Osteoblasts were obtained from newly born rabbit jaw bones and cultured by the method of bone-tissue cultivation. Primary cultivated osteoblast was identified by alkaline phosphatase(ALP) and the mineralization nodes. Zoledronic acid at various concentrations was added to six groups of media with serial subcultivated cells (the final concentration: 0, 10-5, 10-6, 10.7, 10-8 and 10-9 mol/L). At different time, ALP, osteoprotegerin and osteocalcin were observed and calculated.Results The concentration of 10-6,10-7, 10-8 and 10-9 mol/L zoledronic acid significantly increased ALP activity[ (5.91 ± 0. 35 ), ( 7.62 ± 0. 33 ), ( 10. 00 ± 0. 38 ), ( 8. 91 ± 0. 29 ) U/L ]. Protein expression of osteoprotegerin and osteocalcin was enhanced. The differences among the groups were significant (P <0. 01 ). Peak level was attained at a concentration of 10-8 mol/L Conclusions Zoledronic acid promotes osteoblast proliferation and maturation and modulates osteoprotegerin production.