Effects of sodium butyrate on proliferation of human oral squamous carcinoma cell line and expression of p27Kip1
10.3760/cma.j.issn.1002-0098.2010.10.013
- VernacularTitle:丁酸钠对人口腔鳞状细胞癌Tca8113细胞增殖及p27Kip1蛋白表达的影响
- Author:
Ling GONG
1
;
Wen-Mei WANG
;
Yan JI
;
Yu WANG
;
Da-Wei LI
Author Information
1. 南京大学口腔医学院·南京市口腔医院
- Keywords:
Carcinoma,squamous cell;
Cell cycle;
Sodium butyrate;
p27Kip1 protein
- From:
Chinese Journal of Stomatology
2010;45(10):619-622
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of sodium butyrate (NaB) on inhibition of human oral squamous carcinoma cell line. Methods Human oral squamous carcinoma cell line Tca8113 was treated with different concentration of NaB. Light microscope was used to observe the morphological changes of the carcinoma cells. The cell cycle was analyzed by flow cytometry. Expression of p27Kip1 was determined with immunocytochemical assay. ResultsNaB significantly inhibited the proliferation of Tca8113 in a timeand dose-dependent manner. Tca8113 cells treated with NaB was arrested in Go-G1 phase. The fraction of cells in G0-G1 were (63.2 ± 2.4) % and (77.2 ± 3.8) % after treated with NaB at the concentration of 2 and 4 mmol/L alternatively, whereas (48.1 ±2. 4)% in Go-G1 phase were observed in control group(P ＜0. 05). The expression of p27Kip1 was markedly up-regulated after being treated with NaB. Conclusions NaB treatment can inhibit the growth of oral squamous carcinoma cell line in vitro and induce cell cycle arrest, which might be associated with the increased expression of p27Kip1 protein.
