Overexpression and characterization of endo-cellulase A10 from Aspergillus nidulans in Pichia pastoris.

Author: Jianjun LI ¹ ; Xiaolian LI ¹ ; Ziqiang WANG ¹ ; Jianjun LI ¹
Author Information

1. National Key Laboratory of Biochemical Engineering, National Engineering Research Center for Biotechnology (Beijing), Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
Publication Type:Journal Article
Keywords: Aspergillus nidulans; divalent metal ion; endo-cellulase; enzymological properties; fermentation
From: Chinese Journal of Biotechnology 2017;33(5):757-765
CountryChina
Language:Chinese
Abstract: Endo-cellulases are important to efficiently hydrolyze cellulose, and widely used in biotechnology. In this study, we overexpressed and characterized an endo-cellulase from Aspergillus nidulans. This endo-cellulase was successfully overexpressed in flasks and fermentor, and its concentration in fermentor reached 0.89 mg/mL. The optimal pH and temperature of the were 4.0 and 80 ℃ respectively, and it was very stable between pH 2.0 and 12.0. It was thermally stable below 60 ℃, whereas it was inactivated very quickly above 70 ℃. Its CMCase activity could be enhanced by Co²⁺, Mn²⁺ and Fe²⁺, whereas it was inhibited by Pb²⁺, Ni²⁺ and Cu²⁺. Therefore, this endo-cellulase exhibited good pH stability and thermostability below 60 ℃, and has the potential as commercial enzymes.