Cisplatin inhibits proliferation of cervical carcinoma cell line by up-regulating Stat1 expression.
- Author:
Rui-Lin LEI
1
,
2
;
Song-Shu XIAO
;
Min XUE
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents; pharmacology; Cell Proliferation; Cisplatin; pharmacology; Down-Regulation; Drug Resistance, Neoplasm; Female; Gene Expression Regulation, Neoplastic; HeLa Cells; Humans; RNA, Small Interfering; STAT1 Transcription Factor; metabolism; Up-Regulation; Uterine Cervical Neoplasms; pathology
- From: Journal of Southern Medical University 2015;35(1):88-92
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the role of Stat1 gene in the proliferation and chemotherapeutic sensitivity of cervical cancer HeLa cells.
METHODSThe protein expression of Stat1 in the Hela cells exposed to gradient concentrations of cisplatin (DDP) was detected by Western blotting with or without small interfering RNA (siRNA)-mediated Stat1 gene silencing. The effect of Sata1 silencing on the sensitivity to DDP and cell proliferation of the cells was tested using MTT assay and BrdU assay, and the expression of c-Myc was detected by Western blotting in the cells treated with siRNA and DDP.
RESULTSThe expression of Stat1 in Hela cells exposed to DDP increased with the DDP concentrations, reaching 1.5 folds of the baseline at a DDP concentration of 5 mg/L and 2 folds at 10 mg/L. Stat1-siRNA effectively reduced Stat1 expression in Hela cells, promoted the cell proliferation, and enhanced the expression of c-Myc; DDP inhibited the cell growth and down-regulated c-Myc expression. Stat1-siRNA rescued DDP-induced inhibition of cell growth and c-Myc down-regulation.
CONCLUSIONThe expression of Stat1 is associated with DDP sensitivity in cervical cancer cells, and Stat1 silencing can increase the sensitivity to DDP and c-Myc expression of the cells.
