Effect of Radix isatidis polysaccharides on immunological function and expression of immune related cytokines in mice.
- Author:
Yan-ling ZHAO
1
,
2
;
Jia-bo WANG
;
Li-mei SHAN
;
Cheng JIN
;
Li MA
;
Xiao-he XIAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Proliferation; drug effects; Chickens; Cyclophosphamide; pharmacology; Cytokines; blood; metabolism; Drugs, Chinese Herbal; pharmacology; Immunity; drug effects; Immunosuppression; Interferon-gamma; blood; Interleukin-2; blood; Macrophages; cytology; drug effects; Mice; Mice, Inbred BALB C; Phagocytosis; drug effects; Polysaccharides; pharmacology; Spleen; cytology
- From: Chinese journal of integrative medicine 2008;14(3):207-211
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the effect of Radix Isatidis polysaccharides (RIP) on the immunological function and expression of immune related cytokines in mice.
METHODSSixty mice were randomly divided into six groups, the normal group, cyclophosphamide (Cy) model group, Levamisole (positive control) group, RIP low, medium and high dose groups (0.08 g/kg, 0.16 g/kg, 0.32 g/kg, respectively), ten in each group. By detecting the value of abdominal macrophage phagocytic index, serum hemolysin (HC50), proliferation of lymphocytes and expression of related cytokines, interleukin (IL-2) and interferon gamma (INF-gamma), the effect of RIP on immunological function and its mechanism were studied.
RESULTSRIP could improve the level of hemolysin in immunological function depressed mice. The results showed that the value of macrophage phagocytic index in the high dose RIP group increased from 1.11+/-0.13 to 1.42+/-0.26. The level of IL-2 and INF-gamma could be decreased by Cy to 38.12+/-6.88 ng/L and 139.23+/-29.87 ng/L, respectively, while RIP in high dose could increase the secretion of IL-2 and INF-gamma to 53.54+/-14.43 ng/L and 189.91+/-32.63 ng/L, respectively.
CONCLUSIONRIP could enhance non-specific immunological function, humoral immunity and cellular immunity in mice.
