Study on cytotoxic secondary metabolites of endophytic fungus Diaporthe longicolla A616 from Pogostemon cablin.

Mo WANG; Yu-Chan CHEN; Zhang-Hua SUN; Guo-Hui TAN; Hao-Hua LI; Hong-Xin LIU; Han-Jing YAN; Xiao-Ling GUO; Wei-Min ZHANG

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Study on cytotoxic secondary metabolites of endophytic fungus Diaporthe longicolla A616 from Pogostemon cablin.

Author: Mo WANG ¹ ; Yu-Chan CHEN ¹ ; Zhang-Hua SUN ¹ ; Guo-Hui TAN ¹ ; Hao-Hua LI ¹ ; Hong-Xin LIU ¹ ; Han-Jing YAN ² ; Xiao-Ling GUO ² ; Wei-Min ZHANG ¹
Author Information

1. State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangzhou 510070, China.
2. Guangdong Pharmaceutical University, Guangzhou 510006, China.
Publication Type:Journal Article
Keywords: Diaporthe longicolla; Pogostemon cablin; cytotoxic activity; endophytic fungus
From: China Journal of Chinese Materia Medica 2016;41(11):2112-2117
CountryChina
Language:Chinese
Abstract: To study active secondary metabolites of endophytic fungus Diaporthe longicolla A616 isolated from Pogostemon cablin. Ten compounds were isolated from fermentation product of the strain 616 by silica gel, reverse phase silica gel, Sephadex-LH20, HPLC and so on. Their structures were identified as 1,3-diamino-1,3-dimethylurea(1),(7R,9R)-7-hydroxy-9-propyl-5-nonen-9-olide(2), Ergosta-5,7,22-trien-3β-ol(3),(22E,24R)-ergosta-4,6,8(14)-22-tetraen-3-one(4),(22E,24R)-3β,5α-dihydroxy-6β-ergosta-7,22-diene(5), citreoisocoumarin(6), glycerol monolinoleate(7), 1-(2-hydroxyethoxy)ethyl(E)-octadec-9-enoate(8), cyclo-(L-Pro-L-Ala)(9), cyclo(L)-Pro-(L)-Val(10), respectively, based on extensive spectroscopic analysis and literature comparisons. Compounds 6-10 were isolated from the genus Diaporthe for the first time. All isolated compounds were evaluated for in vitro cytotoxic activities against SF-268, MCF-7, NCI-H460 and HepG-2 tumor cell lines. Compounds 4 and 5 showed potent growth inhibitory activities against the four cell lines with IC₅₀ values of 5.3, 6.5, 12.2, 6.1μmol•L⁻¹ and 8.2, 5.2, 6.1, 9.4μmol•L⁻¹, respectively.