Two-dimensional electrophoresis analysis of the protein expression of dental pulp in different conditions
10.3321/j.issn:1002-0098.2008.03.008
- VernacularTitle:不同状态下牙髓组织蛋白质表达的二维电泳分析
- Author:
Chen LIN
1
;
Min NIE
;
Lu ZHANG
;
Zhi CHEN
Author Information
1. 武汉大学口腔医学院
- Keywords:
Dental pulp;
Proteome;
Electrophoresis,gel,two-dimensional;
Mass spectrometry
- From:
Chinese Journal of Stomatology
2008;43(3):154-156
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the different responses of dental pulp to moderate caries and thermal stimulation by proteomies.Methods Two-dimensional electrophoresis(2-DE)was performed to obtain the 2-D gel electrophoresis patterns of dental pulp.Mass spectrometry(MS)was used to analyze several different selected spots in the expression proteins.Results No significant difference in protein expression was found between normal and moderate carious dental.Two protein spots were absent in heatdamaged group and 8 spots showed significantly down-regulated.Seven proteins were identified by MS.Conclusions In the present study,no significant difference in the pulp protein expression was detected between the healthy and moderate carious pulp tissues.However,down-regulation of pulp protein in thermal stimulation was observed.
