Immunolocalization of receptor activator of NF-κB ligand and osteoprotegerin during mouse mandibular first molar eruption
10.3321/j.issn:1002-0098.2008.03.011
- VernacularTitle:核因子κB激活受体的配体和骨保护素在小鼠下颌第一磨牙萌出中的免疫学定位
- Author:
Ling WU
1
;
Fang HUA
;
Xiao-Lei ZHANG
;
Lu ZHANG
;
Zhi CHEN
Author Information
1. 武汉大学口腔医学院
- Keywords:
Tooth eruption;
NF-kappa B;
Osteoprotegerin
- From:
Chinese Journal of Stomatology
2008;43(3):164-167
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the immunolocalization of receptor activator of nuclear factorkappa B ligand(RANKL)and osteoprotegerin(OPG),and to explore the correlation between their expressions and activity of osteoclast during first mandibular molar eruption.Methods Mouse mandibles dissected from postnatal day 1.5 to 14.5 were stained respectively for muhinucleated osteoclasts using tartrate-resistant acid phosphatase(TRAP)staining,and RANKL and OPG protein expression was examined by immunohistochemical staining.Results The two peak values of osteoclast/acrage in the occlusal and basal region were both observed on the P1.5 and P9.5:while the two peak values in the lateral region were on P3.5 and P9.5.During the mouse molar eruption,burst of osteoclastogenesis was associated with high expression of RANKL and low expression of OPG.Conclusions RANKL and OPG could have a close relationship with the osteoclast activity and two developmental apexes were observed during the molar eruption.The occlusal movement was relatively stable,meanwhile the temporarily accelerative movement to the basal and lateral regions could occur.
