Cloning and expression analysis of a key device of HMGR gene involved in ginsenoside biosynthesis of Panax ginseng via synthetic biology approach.
- Author:
Hong-Mei LUO
1
;
Jing-Yuan SONG
;
Xue-Ying LI
;
Chao SUN
;
Chun-Fang LI
;
Xiang LUO
;
Ying LI
;
Shi-Lin CHEN
Author Information
1. National Engineering Laboratory for Breeding of Endangered Medicinal Materials, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
- Publication Type:Journal Article
- MeSH:
Amino Acid Sequence;
Cloning, Molecular;
DNA, Complementary;
genetics;
Flowers;
metabolism;
Gene Expression Regulation, Enzymologic;
Gene Expression Regulation, Plant;
Ginsenosides;
biosynthesis;
Hydroxymethylglutaryl CoA Reductases;
genetics;
metabolism;
Open Reading Frames;
Panax;
genetics;
metabolism;
Phylogeny;
Plant Leaves;
metabolism;
Plant Roots;
metabolism;
Plant Stems;
metabolism;
Plants, Medicinal;
genetics;
metabolism;
Protein Structure, Secondary;
Sequence Homology, Amino Acid;
Synthetic Biology
- From:
Acta Pharmaceutica Sinica
2013;48(2):219-227
- CountryChina
- Language:Chinese
-
Abstract:
3-Hydroxy-3-methylglutaryl coenzyme-A reductase (HMGR), the first enzyme of mavalonic acid pathway, is one of the key devices involved in ginsenoside biosynthesis based on synthetic biology approach. The open reading frame of a novel HMGR gene from Panax ginseng (PgHMGR2) was cloned and analyzed in this study. PgHMGR2-encoding protein showed 71.6% sequence similarity to a P. ginseng HMGR in GenBank. The full-length cDNA sequence of PgHMGR2 containing 1 770 bp, which encodes 589 amino acids, was cloned by RT-PCR strategy from P. ginseng. The bioinformatic analysis showed that PgHMGR2-encoding protein contained two transmembrane regions and the HMG_CoA_reductase domain, without signal peptide. The protein sequence of PgHMGR2 had the highest sequence similarity (99%) with Panax quinquefolius HMGR (GenBank accession No. ACV65036). The expression level of PgHMGR2 was the highest in flower based on a real-time PCR analysis, followed by leaf and root, and the lowest was in stem. The result will provide a foundation for exploring the molecular function of PgHMGR2 involved in ginsenoside biosynthesis based on synthetic biology approach in P. ginseng plants.
