A 1H NMR based metabonomics approach to progression of coronary atherosclerosis in a hamster model.
- Author:
Chun-Ying JIANG
1
;
Kang-Min YANG
;
Liu YANG
;
Zhao-Xia MIAO
;
Ying-Hong WANG
;
Hai-Bo ZHU
Author Information
1. (State Key Laboratory ofBioactive Substances and Functions of Natural Medicines, Beijing Key Laboratory of New Drug Mechanisms and Pharmacological Evaluation, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
- Publication Type:Journal Article
- MeSH:
Amino Acids;
blood;
Animals;
Biomarkers;
metabolism;
Cholesterol;
blood;
Coronary Artery Disease;
blood;
metabolism;
Disease Models, Animal;
Disease Progression;
Lipids;
blood;
Magnetic Resonance Spectroscopy;
Male;
Mesocricetus;
Metabolome;
Metabolomics;
Oxidative Stress;
drug effects;
Principal Component Analysis;
Random Allocation
- From:
Acta Pharmaceutica Sinica
2013;48(4):495-502
- CountryChina
- Language:Chinese
-
Abstract:
To obtain a better understanding of the progression of atherosclerosis and identify potential biomarkers, proton nuclear magnetic resonance spectroscopy (1H NMR)-based metabonomics was used to study the metabolic changes in the plasma of hamster fed with a high-fat/cholesterol diet. Plasma samples were collected at different time points during the progression of atherosclerosis and individual proton NMR spectra were visually and statistically assessed using multivariate analyses. NMR results for all samples showed a time-dependent development from physiological to pathophysiological status during atherosclerosis. Analysis of the identified biomarkers of atherosclerosis suggests that lipid and amino acid metabolisms are significantly disturbed, together with inflammation, oxidative stress, following cholesterol overloading. The results enriched our understanding of the mechanism of atherosclerosis and demonstrated the effectiveness of the NMR-based metabonomics approach to study such a complex disease.
