Establishment of immortalized lymphoblastoid cell bank of a keloid pedigree.
- Author:
Xin YAN
1
;
Jian-hua GAO
;
Mei SONG
;
Xiao-jun LIU
;
Yang CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Aged; Cell Line, Transformed; Cell Transformation, Viral; Child; Child, Preschool; Cryopreservation; Female; Herpesvirus 4, Human; physiology; Humans; Keloid; blood; genetics; Lymphocytes; pathology; virology; Male; Middle Aged; Pedigree
- From: Journal of Southern Medical University 2006;26(1):22-24
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate a method for establishing immortalized lymphoblastoid cell bank of keloid pedigree so as to provide a long-term source of specimens for keloid research.
METHODSWith Epstein-Barr virus transformation, fresh and frozen blood samples collected from all members of the keloid pedigree were used respectively to establish the immortalized lymphoblastoid cell lines of B lymphocytes.
RESULTSTwenty-seven immortalized lymphoblastoid cell lines of the keloid pedigree were obtained successfully, and all cell lines survived cryopreservation in liquid nitrogen.
CONCLUSIONSThe immortalized lymphoblastoid cell bank of the keloid pedigree can preserve the whole gene information and provide long-term DNA sources for keloid research. Preparation of the cell lines with fresh blood is more efficient than that with frozen blood.
