Effects of celecoxib on PGE2 synthesis and COX-2 and VEGF-C mRNA expression in Tca8113 cell lines.
- Author:
Wei-Zhong LI
1
;
Xiao-Yan WANG
;
Yan-Qing DING
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Squamous Cell; blood supply; metabolism; pathology; Celecoxib; Cell Line, Tumor; Cyclooxygenase 2; genetics; metabolism; Cyclooxygenase 2 Inhibitors; pharmacology; Dinoprostone; metabolism; Humans; Pyrazoles; pharmacology; RNA, Messenger; genetics; metabolism; Sulfonamides; pharmacology; Tongue Neoplasms; blood supply; metabolism; pathology; Vascular Endothelial Growth Factor C; genetics; metabolism
- From: Journal of Southern Medical University 2009;29(3):466-468
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of the selective cyclooxygenase-2 (COX-2) inhibitor celecoxib on prostaglandin E(2) (PGE2) release and vascular endothelial growth factor C (VEGF-C) and COX-2 mRNA expression in Tca8113 cell lines.
METHODSMTT assay was used to analyze the proliferation of Tca8113 cells. The PGE2 level was detected with enzyme-linked immunosorbent assay (ELISA), and the expressions of COX-2 and VEGF-C mRNA were examined with RT-PCR.
RESULTSCelecoxib could induce inhibitory effects on the growth and PGE2 release in Tca8113 cells. The RT-PCR results showed that celecoxib significantly down-regulated the expression of VEGF-C mRNA, but produced a weak effect on COX-2 mRNA expression.
CONCLUSIONThe inhibitory effect of celecoxib on Tca8113 cell growth and the expressions of VEGF-C and COX-2 may be related to the release of PGE2.
