Purification, characterization and production optimization of a vibriocin produced by mangrove associated Vibrio parahaemolyticus.

Baskar BALAKRISHNAN; Jayappriyan Kothilmozhian RANISHREE; Sathish THADIKAMALA; Prabakaran PANCHATCHARAM

Return

Purification, characterization and production optimization of a vibriocin produced by mangrove associated Vibrio parahaemolyticus.

Author: Baskar BALAKRISHNAN ¹ ; Jayappriyan Kothilmozhian RANISHREE ² ; Sathish THADIKAMALA ² ; Prabakaran PANCHATCHARAM ³
Author Information

1. Center for Bioenergy, Cooperative research, Lincoln University, Jefferson City, Missouri 65101, USA ; Marine Biotechnology, Andaman and Nicobar Centre for Ocean Science and Technology, National Institute of Ocean Technology, PortBlair, Andaman and Nicobar Islands 744103, India ; Department of Microbiology, PRIST University, Thanjavur, Tamil Nadu 614904, India.
2. Marine Biotechnology, Andaman and Nicobar Centre for Ocean Science and Technology, National Institute of Ocean Technology, PortBlair, Andaman and Nicobar Islands 744103, India.
3. Department of Microbiology, PRIST University, Thanjavur, Tamil Nadu 614904, India.
Publication Type:Journal Article
Keywords: Antimicrobial peptide; Mangrove rhizosphere; Vibrio harveyi; Vibrio parahaemolyticus; Vibriocin
From:Asian Pacific Journal of Tropical Biomedicine 2014;4(4):253-261
CountryChina
Language:English
Abstract:
OBJECTIVETo identify a potential bacterium which produces antimicrobial peptide (vibriocin), and its purification, characterization and production optimization. The bacteria subjected in the study were isolated from a highly competitive ecological niche of mangrove ecosystem.
METHODSThe bacterium was characterized by phenotype besides 16S rRNA gene sequence analysis. The antibacterial activity was recognised by using agar well diffusion method. The vibriocin was purified using ammonium sulphate precipitation, butanol extraction, gel filtration chromatography, ion-exchange chromatography and subsequently, by HPLC. Molecular weight of the substance identified in SDS-PAGE. Production optimization performed according to Taguchi's mathematical model using 6 different nutritional parameters as variables.
RESULTSThe objective bacterium was identified as Vibrio parahaemolyticus. The vibriocin showed 18 KDa of molecular mass with mono peptide in nature and highest activity against pathogenic Vibrio harveyi. The peptide act stable in a wide range of pH, temperature, UV radiation, solvents and chemicals utilized. An overall ∼20% of vibriocin production was improved, and was noticed that NaCl and agitation speed played a vital role in secretion of vibriocin.
CONCLUSIONThe vibriocin identified here would be an effective alternative for chemically synthesized drugs for the management of Vibrio infections in mariculture industry.