RNA interference inhibits the expression of survivin and induces the apoptosis of PC-3 cells.
- Author:
Yi-bing WANG
1
;
Yi-li LIU
;
Ping WANG
;
Xiao-liang FU
;
Zhi-xi SUN
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Cell Line, Tumor; Gene Expression Regulation, Neoplastic; Humans; Inhibitor of Apoptosis Proteins; Lipids; chemistry; Male; Microtubule-Associated Proteins; genetics; Neoplasm Proteins; genetics; Plasmids; chemistry; genetics; Prostatic Neoplasms; genetics; pathology; RNA Interference; RNA, Messenger; genetics; metabolism; RNA, Small Interfering; genetics; Reverse Transcriptase Polymerase Chain Reaction; Transfection; methods
- From: National Journal of Andrology 2007;13(11):983-987
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the inhibitory effect of RNA interference (RNAi) on the expression of survivin mRNA and its inducibility of the apoptosis of PC-3 cells.
METHODSsiRNA expression vectors were designed and constructed to be directed at survivin and transfected into PC-3 cells by Lipofectamine in 4 groups: plasmid A, plasmid B, negative sequence and control E. RT-PCR, Western blot and flow cytometry were used to detect the expression of survivin mRNA and the apoptosis of PC-3 cells.
RESULTSThe expression rates of survivin protein were 18.94% +/- 0.63%, 16.35 +/- 0.23%, 46.41% +/- 0.76% and 46.20 +/- 1.47%, those of survivin mRNA were 27.94% +/- 1.43%, 24.51% +/- 1.37%, 49.46% +/- 0.71% and 48.49% +/- 1.32%, and the apoptosis rates of PC-3 cells were 12.80% +/- 1.33%, 16.48% +/- 1.00%, 3.03% +/- 0.62% and 2.96% +/- 0.41% respectively in different groups.
CONCLUSIONRNAi can effectively inhibit the expression of survivin mRNA and induce the apoptosis of PC-3 cells.
