Construction of vectors expressing M2 and NA genes of H5N1 influenza virus

VernacularTitle:H5N1亚型流感病毒M2与NA基因真核表达载体的构建与鉴定
Author: Jian-Qiang GUO ¹ ; Li-Hong YAO ; Ai-Jun CHEN ; Xiao-Yu LIU ; Jin-Qi FU ; Peng-Wei XU ; Zhi-Qing ZHANG
Author Information

1. 中国疾病预防控制中心病毒病预防控制所
Keywords: Influenza A virus; Viral proteins; Neuraminidase; Expressed sequence tage
From: Chinese Journal of Experimental and Clinical Virology 2011;25(3):167-169
CountryChina
Language:Chinese
Abstract: Objective To construct vectors expressing M2 and NA genes of H5N1 influenza virus. Methods Based on the human HSN1 avian influenza virus ( A/Anhui/1/2005) isolated in china, M2 and NA genes were amplified by PCR. M2 or NA gene was subcloned into pStar vector to construct recombinant pStar-M2/, pStar-/M2, pStar-NA/and pStar-NA/. Furthermore, both of the M2 and NA genes were subcloned into pStar to construct two genes co-expressing recombinant pStar-M2/NA and pStar-NA/M2. Expression of the genes were detected by IFA after transfection of 293 cells with the recombinant plasmids. Results Recombinant plasmids were constructed and identified by restriction endonuclease digestion. Expression of the genes cloned into the recombinant plasmids was confirmed by IFA. Conclusion Recombinant plasmids expressing M2 and/or NA genes of H5N1 influenza virus were constructed, which provided basis for development of influenza DNA vaccine.