Construction and recovery of chimeric rabies virus expressing envelop proteins E1E2 of hepatitis C virus
10.3760/cma.j.issn.1003-9279.2011.03.004
- VernacularTitle:狂犬/丙型肝炎嵌合病毒的构建和拯救
- Author:
Jia-Liang DU
1
;
Ying HUANG
;
Qing TANG
;
Li-Hua WANG
;
Guo-Dong LIANG
Author Information
1. 中国疾病预防控制中心病毒病预防控制所
- Keywords:
Rabies virus;
Hepatitis C;
Hepatitis viruses;
Vaccines,virosome
- From:
Chinese Journal of Experimental and Clinical Virology
2011;25(3):170-172
- CountryChina
- Language:Chinese
-
Abstract:
Objective Construction and recovery of chimeric rabies virus expressing HCV envelop proteins E1E2. Methods On the basis of the previously established reverse genetic system CTN-CFP, HCV E1 E2 genes were cloned to both replication competent and replication constrained viral vectors based on CTN181 strain and the chimeric viruses CTN-HCV E1E2 and CTNAG-HCV E1E2 were recovered. Results The result demonstrated that both the chimeric viruses were rescued successfully, had the ability to reinfect normal sensitive cell lines and express HCV E1E2 genes detected in the level of mRNA. Conclusion The establishment of chimeric RVs expressing HCV E1E2 genes provides the evidence that it is feasible to develop novel HCV vaccines based on viral vectors in theory and in practice.
