Effect of Qishen Yiqi droplet on adenylate contents in myocardium of cor pulmonale rats.
10.19540/j.cnki.cjcmm.20161222.022
- Author:
Shi-Han GENG
1
;
Qiu-Yue SHAN
2
;
Meng-Xi XU
1
;
Yi-Dan ZHANG
1
;
Li-Li SONG
1
;
Ning HOU
1
;
Wen ZHANG
2
;
Mi-Xia ZHANG
1
;
Peng-Wei ZHUANG
1
;
Yan-Jun ZHANG
1
Author Information
1. Tianjin Key Laboratory of Traditional Chinese Medicine Pharmacology, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
2. Tasly Group Research Institute, Tianjin 300410, China.
- Publication Type:Journal Article
- Keywords:
HPLC;
Qishen Yiqi droplet;
content determination;
cor pulmonale;
energy metabolism
- From:
China Journal of Chinese Materia Medica
2017;42(1):170-174
- CountryChina
- Language:Chinese
-
Abstract:
Cor pulmonale rat models were induced by a single intraperitoneal injection of monocrotaline(MCT), and the sham group received a single intraperitioneal injection of normal saline. After the model rats received intragastric administration of Qishen Yiqi droplet(QS) for 6 weeks, the contents of adenylate(ATP, ADP and AMP) in right myocardial tissues were measured by HPLC, and then the metabolism changes in myocardium of cor pulmonale rats with QS were investigated. The results showed that ATP, ADP, and AMP were well separated, with a good linearity within a certain range of concentration; and the recovery rates were within the range of 90%-108%. As compared with model group, the level of ATP was significantly elevated in high-dose treatment group; ADP contents showed an increasing trend and AMP contents showed a decreasing trend, indicating that QS could significantly improve energy metabolism system in myocardium. By using the HPLC, a qualitative and quantitative analysis method was given for the determination of ATP, ADP and AMP contents in myocardium, providing a method for energy metabolism measurement in biological samples.
