Protective effects of LBP on cerebral ischemia reperfusion injury in mice and mechanism of inhibiting NF-κB, TNF-α, IL-6 and IL-1β.
10.19540/j.cnki.cjcmm.20161222.016
- Author:
Jian-Bin GE
1
;
Hong-Jian LU
1
;
Xin-Jian SONG
1
;
Mei LI
2
;
Dan-Dan CHEN
3
;
Feng WU
3
Author Information
1. The Second People's Hospital of Nantong, Nantong 226002, China.
2. Department of Pharmacology, College of Pharmaceutical Science, Soochow University, Suzhou 215123, China.
3. Department of Pharmacology, Nantong University, Nantong 226001,China.
- Publication Type:Journal Article
- Keywords:
LBP;
cerebral ischemia;
interleukin-1β;
interleukin-6;
nuclear factor κB;
tumor necrosis factor-α
- From:
China Journal of Chinese Materia Medica
2017;42(2):326-331
- CountryChina
- Language:Chinese
-
Abstract:
To observe the protective effects of Lycium barbarum polysaccharides (LBP) on cerebral ischemia reperfusion injury in mice and explore its mechanism. Common carotid artery thread was used to cause middle cerebral artery ischemia, and the thread was taken out after 2 h ischemia to achieve cerebral ischemia reperfusion injury in mice. Therefore, the transient middle cerebral artery occlusion (tMCAO) models were established to observe the effects of LBP (25,50, 100 mg•kg⁻¹) on neurological outcome, infarct size and water contents. HE staining was used to observe its effects on neurocytes of cerebral tissues in mice. Western blotting was used to evaluate the protein expression levels of NF-κB p65. ELISA was used to evaluate the levels of TNF-α, IL-6 and IL-1β in the serum. According to the results, LBP markedly improved neurologic deficits, and decreased infarct size and water contents at 24 h after reperfusion in mice. Pathological section of brain tissues also proved its protective effects on neurocytes. Western blot analysis indicated that LBP markedly down-regulated the protein level of NF-κB p65. ELISA indicated that LBP decreased the levels of TNF-α, IL-6 and IL-1β in the serum 24 h after reperfusion.In conclusion, LBP has protective effects on cerebral ischemia reperfusion injury in mice, and this effect may be associated with inhibiting NF-κB and inflammatory reactions.
