The Role of the Glutamate Receptor in the Transient Focal Cerebral Ischemia in Rats.
10.4097/kjae.2000.38.6.1062
- Author:
Chang Hee CHAE
1
;
Hae Kyu KIM
;
Kyung Hoon KIM
;
Seong Wan BAIK
;
Inn Se KIM
Author Information
1. Department of Anesthesiology, College of Medicine, Pusan National University, Pusan, Korea.
- Publication Type:Original Article
- Keywords:
Animal: rats;
Brain: edema;
middle cerebral artery occlusion;
Pharmacology: penty lenetetrazole;
MK-801
- MeSH:
Animals;
Brain Ischemia*;
Dizocilpine Maleate;
Glutamic Acid*;
Humans;
Infarction;
Infarction, Middle Cerebral Artery;
Male;
Neurons;
Pentylenetetrazole;
Rats*;
Rats, Sprague-Dawley;
Receptors, Glutamate*
- From:Korean Journal of Anesthesiology
2000;38(6):1062-1067
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: To investigate the role of glutamate in the transient focal cerebral ischemia, a reversible middle cerebral artery occlusion model was induced in 25 male Sprague-Dawley rats. METHODS: Triphenyltetrazolium chloride (TTC) stain was used for evaluation of the changes of infarction ratio in MK-801 (0.3, 1.0, 3.0 mg/kg) or pentylenetetrazole (50 mg/kg) treated groups. RESULT: The infarction ratio at 48 hours after 2 hour transient focal brain ischemia was 39.2 +/- 13.2% in control group and 23.8 +/- 4.2, 27.0 +/- 8.9, and 12.8 +/- 4.4% in MK-801 (0.3, 1.0, 3.0 mg/kg) groups. In the pentylenetetrazole (PTZ) group, the infarction ratio was 32.6 +/- 6.7%. CONCLUSIONS: The non-specific glutamate receptor antagonist, MK-801, showed a trend toward dose-dependent improvement, but the PTZ group showed no improvement. From these results, it suggested that glutamate might be partly involved in the mechanisms of ischemia-induced neuronal damage.
