In Vivo Cartilage Formation Using Human Bone Marrow-Derived Mesenchymal Stem Cells Mixed with Fibrin Glue.
- Author:
Ki Won PARK
1
;
Woo Jin CHOI
;
Kyoung Mi LEE
;
Dong Suk YOON
;
Sung Hwan KIM
;
Jin Woo LEE
Author Information
1. Brain Korea 21 Project Plus for Medical Science, Yonsei University College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Human bone marrow-derived Mesenchymal stem cells;
Cartilage;
Chondrogenesis;
TGF-beta3;
Fibrin glue
- MeSH:
Animals;
Cartilage*;
Chondrogenesis;
Collagen Type II;
Fibrin Tissue Adhesive*;
Fibrin*;
Humans*;
Mesenchymal Stromal Cells*;
Mice;
Mice, Nude;
Real-Time Polymerase Chain Reaction;
Transforming Growth Factor beta3
- From:Journal of Korean Orthopaedic Research Society
2015;18(2):43-50
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To investigate the feasibility of in vivo cartilage formation by direct injection the chondrogenic undifferentiated human bone marrow-derived mesenchymal stem cells (hBMSCs) mixed with fibrin glue including TGF-beta3. MATERIALS AND METHODS: Chondrogenic differentiation induced hBMSCs for 14 days (control group-2) and undifferentiated hBMSCs combined with TGF-beta3 mixed (experimental group-3) with fibrin glue and fibrin glue only (control group-1) were injected subcutanteously into the back of nude mouse. For evaluation of the cartilage-like tissue formed after 8 weeks after injection, real time PCR, histological analysis and immunohistochemical analysis were used. RESULTS: Control group-1 did not form any visible mass. Control group-2 as well as experimental group-3 could form new cartilage-like tissue which were demonstrated expression of type II collagen by real-time PCR, histology analysis such as H&E staining, MT staining and type II collagen specific immunohistologic analysis. As results, expression of type II collagen was shown in the both groups. CONCLUSION: Our study confirmed that cartilage-like tissues could be formed in subcutaneous layer of nude mouse by direct injection mixed with fibrin glue including TGF-beta3 without chondrogenic-induction of hBMSCs, suggesting that these model could be suitable for preliminary studies or optimizing experiments to evaluate reconstruction of cartilage.
