Changes of Nitric Oxide and Cell Proliferation after Bacillus Calmette-Guerin and Nitric Oxide Synthase Inhibitor Treatment on Murine Bladder Tumor-2 Cell Line.
- Author:
Soo Bang RYU
1
;
Bong Ryoul OH
;
Taek Won KANG
;
Sang Hyun OH
;
Hee Sam NAH
;
Hyun Chul LEE
Author Information
1. Department of Urology, Chonnam National University Medical School, Gwangju, Korea. sbryu@chonnam.ac.kr
- Publication Type:Original Article
- Keywords:
Bacillus Calmette-Guerin;
MBT-2 cell line;
Nitric oxide;
Nitric oxide synthase inhibitor;
Apoptosis
- MeSH:
Apoptosis;
Bacillus*;
Cell Cycle;
Cell Death;
Cell Line*;
Cell Proliferation*;
Mycobacterium bovis;
NG-Nitroarginine Methyl Ester;
Nitric Oxide Synthase*;
Nitric Oxide*;
Up-Regulation;
Urinary Bladder*
- From:Korean Journal of Urology
2003;44(2):155-159
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: We hypothesize that the cytolytic activity of Bacillus Calmette-Guerin (BCG) may act through nitric oxide (NO) production, and that cell death is correlated with apoptosis, changes of NO and cell proliferation following BCG and/or nitric oxide synthase (NOS) inhibition treatment of a murine bladder tumor-2 (MBT-2) cell line. If these cell lines show cell death due to apoptosis was also determined. MATERIALS AND METHODS: NO production and proliferation activity of the MBT-2 cell line were measured after stimulation, with BCG and/or L-NAME, using an enzyme- linked immunosorbent assay (ELISA). After incubation of the MBT-2 cells with BCG and/or L-NAME, the cell cycle was analysis was performed by immunocytometry. RESULTS: The production of NO in the MBT-2 cells was significantly increased by the BCG. The BCG had direct dose-dependent cytotoxic effects on the MBT-2 cell line. After the L-NAME treatment, both the NO production and cytotoxicity were decreased (p<0.05). When the MBT-2 cells were cultured with BCG, the apoptotic cell ratio increased compared to that of the MBT-2 cells treated with L-NAME (p<0.05). CONCLUSIONS: The up-regulation of the production of NO following BCG treatment of the MBT-2 cell line may be due, in part, to the cytolytic action of the BCG. The cell death, when BCG was instilled, correlated with apoptosis.
