The Effect of Lipo-PGE1 on the Viability of Injected Adipose Tissue on Mouse Model.
- Author:
Eui Sun OH
1
;
Ji Eun JUNG
;
Dae Hyun LEW
Author Information
1. Institute for Human Tissue Restoration & Department of Plastic & Reconstructive Surgery, Yonsei University College of Medicine, Seoul, Korea. dhlwq@yuhs.ac
- Publication Type:Original Article
- Keywords:
Fat graft;
Lipo-PGE1;
Survival rate;
Histology
- MeSH:
Absorption;
Adipose Tissue;
Alprostadil;
Animals;
Humans;
Inflammation;
Lipectomy;
Mice;
Mice, Inbred ICR;
Scalp;
Survival Rate;
Transplants
- From:Journal of the Korean Society of Plastic and Reconstructive Surgeons
2010;37(5):526-530
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: With the recent recognition of the importance of soft-tissue fillers, fat grafting has been assumed an increasingly important role as both an adjunctive and a primary procedure in aesthetic and reconstructive surgery. The main problem in achieving long-term soft-tissue augmentation is partial absorption of the injected fat and hence the need for overcorrection and re-injection. The purpose of this study is to improve the viability of the injected fat by the use of Lipo-PGE1. METHODS: Human adipose tissue, obtained by suction-assisted lipectomy, was re-injected into the subcutaneous layer in the scalp of ICR mice. Lipo-PGE1 (0.5 microgram/kg) was injected intravenously in experimental group for 7 days from the operation day and saline was injected in control group. There were 5 animals in each group. The animals were euthanized 4 weeks after the procedure. Graft weight and volume were measured and histologic evaluation was performed. RESULTS: Histologic analysis demonstrated significantly less cyst formation and less inflammatory reaction in the group treated with Lipo-PGE1. No significant difference was found between the groups regarding graft volume or the other histologic parameters investigated. Significant differences were demonstrated in microvascular density count. CONCLUSION: Less cyst formation, less inflammation, more angiogenesis indicating improved quality of the injected fat can be obtained by the addition of Lipo-PGE1. Further studies of various dosages of Lipo-PGE1 and their long-term effect are required before these encouraging results could be applied clinically.
