Epigallocatechin-3-gallate Inhibits LPS-Induced NF-kappaB and MAPK Signaling Pathways in Bone Marrow-Derived Macrophages.
- Author:
So Young JOO
1
;
Young A SONG
;
Young Lan PARK
;
Eun MYUNG
;
Cho Yun CHUNG
;
Kang Jin PARK
;
Sung Bum CHO
;
Wan Sik LEE
;
Hyun Soo KIM
;
Jong Sun REW
;
Nack Sung KIM
;
Young Eun JOO
Author Information
1. Department of Internal Medicine, Chonnam National University Medical School, Gwangju, Korea. yejoo@chonnam.ac.kr
- Publication Type:Original Article
- Keywords:
Epigallocatechin-3-gallate;
Nuclear factor-kappaB;
Mitogen-activated protein kinase;
Macrophage
- MeSH:
Blotting, Western;
Butadienes;
Catechin;
Fluorescent Antibody Technique;
Gene Expression;
I-kappa B Proteins;
Intercellular Adhesion Molecule-1;
Interleukin-12 Subunit p40;
Interleukin-6;
Macrophages;
NF-kappa B;
Nitriles;
Phosphorylation;
Protein Kinases;
RNA, Messenger;
Tea;
Vascular Cell Adhesion Molecule-1
- From:Gut and Liver
2012;6(2):188-196
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND/AIMS: Epigallocatechin-3-gallate (EGCG), the primary catechin in green tea, has anti-inflammatory and anti-oxidative properties. The aim of the current study was to characterize the impact of EGCG on lipopolysaccharide (LPS)-induced innate signaling in bone marrow-derived macrophages (BMMs) isolated from ICR mice. METHODS: The effect of EGCG on LPS-induced pro-inflammatory gene expression and nuclear factor-kappaB (NF-kappaB) and mitogen-activated protein kinase (MAPK) signaling was examined using reverse transcription-polymerase chain reaction, Western blotting, immunofluorescence, and the electrophoretic mobility shift assay. RESULTS: EGCG inhibited accumulation of LPS-induced IL-12p40, IL-6, MCP-1, ICAM-1, and VCAM-1 mRNA in BMMs. EGCG blocked LPS-induced IkappaBalpha degradation and RelA nuclear translocation. EGCG blocked the DNA-binding activity of NF-kappaB. LPS-induced phosphorylation of ERK1/2, JNK, and p38 was inhibited by EGCG. U0126 (an inhibitor of MEK-1/2) suppressed the LPS-induced IL-12p40, IL-6, MCP-1, ICAM-1, and VCAM-1 mRNA accumulation in BMMs. CONCLUSIONS: These results indicate that EGCG may prevent LPS-induced pro-inflammatory gene expression through blocking NF-kappaB and MAPK signaling pathways in BMMs.
