Interaction between Trichomonas vaginalis and the Prostate Epithelium.
10.3347/kjp.2017.55.2.213
- Author:
Jung Hyun KIM
1
;
Ik Hwan HAN
;
Sang Su KIM
;
Soon Jung PARK
;
Duk Young MIN
;
Myoung Hee AHN
;
Jae Sook RYU
Author Information
1. Department of Environmental Biology and Medical Parasitology, Graduate School of Biomedical Science and Engineering, Hanyang University College of Medicine, Seoul 04763, Korea. jsryu@hanyang.ac.kr
- Publication Type:Brief Communication
- Keywords:
Trichomonas vaginalis;
epithelial cell;
cell adhesion;
inflammation
- MeSH:
Cadherins;
Cell Adhesion;
Epithelial Cells;
Epithelial-Mesenchymal Transition;
Epithelium*;
Humans;
Inflammation;
Interleukin-6;
Male;
Prostate*;
Trichomonas vaginalis*;
Trichomonas*;
Vimentin
- From:The Korean Journal of Parasitology
2017;55(2):213-218
- CountryRepublic of Korea
- Language:English
-
Abstract:
Most men infected with Trichomonas vaginalis are asymptomatic and can remain undiagnosed and untreated. This has been hypothesized to result in chronic persistent prostatic infection. Adhesion of the protozoan organisms to mucosal cells is considered a first and prerequisite step for T. vaginalis infection. Adhesion of T. vaginalis to prostate epithelial cells has not yet been observed; however, there are several reports about inflammation of prostate epithelial cells induced by T. vaginalis. The aim of this study was to investigate whether adhesion and cytotoxicity of T. vaginalis are involved in inflammation of prostate epithelial cells. When RWPE-1 cells were infected with T. vaginalis (1:0.4 or 1:4), adhesion of T. vaginalis continuously increased for 24 hr or 3 hr, respectively. The cytotoxicity of prostate epithelial cells infected with T. vaginalis (RWPE-1: T. vaginalis=1:0.4) increased at 9 hr; at an infection ratio of 1:4, cytotoxicity increased after 3 hr. When the RWPE-1 to T. vaginalis ratio was 1:0.4 or 1:4, production of IL-1β, IL-6, CCL2, and CXCL8 also increased. Epithelial-mesenchymal transition (EMT) was verified by measuring decreased E-cadherin and increased vimentin expression at 24 hr and 48 hr. Taken together, the results indicate that T. vaginalis adhered to prostate epithelial cells, causing cytotoxicity, pro-inflammatory cytokine production, and EMT. Our findings suggest for the first time that T. vaginalis may induce inflammation via adhesion to normal prostate epithelial cells.
