Curcumin Attenuates Nuclear Factor-kappaB, c-Jun N-Terminal Kinase and p38 in Tumor Necrosis Factor-alpha-Stimulated Endothelial Cells.
10.4070/kcj.2006.36.7.482
- Author:
Yong Sook KIM
1
;
Youngkeun AHN
;
Moon Hwa HONG
;
Soo Yeon JOO
;
Kye Hun KIM
;
Il Suk SOHN
;
Hyung Wook PARK
;
Young Joon HONG
;
Ju Han KIM
;
Weon KIM
;
Myung Ho JEONG
;
Jeong Gwan CHO
;
Jong Chun PARK
;
Jung Chaee KANG
Author Information
1. The Heart Center of Chonnam National University Hospital, Gwangju, Korea. cecilyk@hanmail.net
- Publication Type:Original Article
- Keywords:
Curcumin;
Nuclear factor-kappaB;
Tumor necrosis factor-alpha;
Inflammation;
Endothelial cell
- MeSH:
Antioxidants;
Blotting, Western;
Cell Adhesion;
Curcuma;
Curcumin*;
Endothelial Cells*;
Enzyme-Linked Immunosorbent Assay;
Human Umbilical Vein Endothelial Cells;
Immunohistochemistry;
Inflammation;
Interleukins;
JNK Mitogen-Activated Protein Kinases*;
Monocytes;
Necrosis*;
NF-kappa B;
Phosphorylation;
Protein Kinases;
Reactive Oxygen Species;
Transducers;
Tumor Necrosis Factor-alpha
- From:Korean Circulation Journal
2006;36(7):482-489
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND AND OBJECTIVES: Curcumin, a yellow pigment of turmeric in curry, has been reported to interfere with nuclear factor (NF)-kappaB. This study was designed to investigate the underlying pathway of the anti-inflammation effect of curcumin on endothelial cells. MATERIALS AND METHODS: Human umbilical vein endothelial cells (HUVECs) were stimulated with tumor necrosis factor (TNF)-alpha (10 ng/mL). The levels of intracellular reactive oxygen species (ROS) were examined using a fluorescent dye DCFH-DA, and the adhesion of U-937 monocytes to the HUVECs was then examined. Nuclear factor kappa B (NF-kappaB) activation was determined by the NF-kappaB p65 translocation to the nucleus via immunocytochemistry. The expression of the NF-kappaB dependent pro-inflammatory molecules were measured by RT-PCR and ELISA. The phosphorylations of c-Jun N-terminal protein kinase (JNK), p38 and STAT-3 (signal transducer and activator of transcription-3) were measured by Western blotting. RESULTS: Curcumin blocked the activation of NF-kappaB by TNF-alpha, and it also reduced the ROS, monocyte adhesion and the phosphorylation of JNK, p38 and STAT-3 in the TNF-alpha-stimulated HUVECs. The expression of intracellular cell adhesion molecule (ICAM)-1, monocyte chemoattractant protein (MCP)-1, and interleukin (IL)-8 were attenuated by curcumin at both the transcription and translation levels. CONCLUSION: We suggest that curcumin could contribute to protection against the adverse vascular effects of the pro-inflammatory response through the modulation of NF-kappaB, JNK, p38 and STAT-3, and this is in addition to its antioxidant effect in endothelial cells.
