Change of Chemosensitivity and Cellular Characteristics of Cisplatinum Resistant Human Bladder Cancer Cells Induced by Long-term Cisplatinum Treatment.
- Author:
Eunsik LEE
1
;
Kyung Joon MIN
;
Soo Woong KIM
;
Hae Won LEE
;
Kyu Seo CHO
;
Kwang Myung KIM
;
Sang Eun LEE
;
Chongwook LEE
Author Information
1. Department of Urology, Seoul National University College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Bladder neoplasms;
Cell line;
Cisplatinum;
Drug-resistance
- MeSH:
Cell Cycle;
Cell Line;
DNA;
Humans*;
Parents;
Urinary Bladder Neoplasms*;
Urinary Bladder*
- From:Korean Journal of Urology
1998;39(4):305-310
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Cisplatinum has the therapeutic efficacy against a bladder cancer. However, the response is often limited due to appearance of drug-resistant tumor cells. The studies of establishment and characterization of cisplatinum-resistant tumor cells are considered to be helpful in elucidating the underlying mechanism of acquired resistance to cisplatinum in human tumors. MATERIALS AND METHODS: We established cisplatinum-resistant cell lines sequentially, T24Rl and T24R2, which show resistance to cisplatinum at a concentration of 1 and 2microgram /ml, respectively, by the stepwise exposure of T24 human bladder cancer cell to increasing concentrations of cisplatinum. RESULTS: The resistance to cisplatinum of T24Rl and T24R2 cells was 13- and 18-fold that of the parental T24 cells, respective1y. Growth, DNA synthesis and cell cycle distribution of T24Rl and T24R2 cells were not different from those of the parental T24 cells. CONCLUSIONS: These cisplatinum-resistant bladder tumor cell lines could be a useful model to elucidate the biochemical and molecular mechanism Involved In the cisplatinum resistance.
