Combination Effect of Nimotuzumab with Radiation in Colorectal Cancer Cells.
10.3857/jkstro.2010.28.3.147
- Author:
Hye Kyung SHIN
1
;
Mi Sook KIM
;
Jae Hoon JEONG
Author Information
1. Division of Radiation Cancer Research, Korea Institute of Radiological and Medical Sciences, Seoul, Korea. jeongj@kirams.re.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
Colorectal cancer;
EGFR;
Nimotuzumab;
Radiation;
Combined therapy
- MeSH:
Antibodies, Monoclonal, Humanized;
Blotting, Western;
Cell Cycle;
Cell Line;
Cell Proliferation;
Cell Survival;
Colorectal Neoplasms;
Flow Cytometry;
Humans;
Phosphorylation;
Radiation Tolerance;
Radiation-Sensitizing Agents;
Receptor, Epidermal Growth Factor
- From:The Journal of the Korean Society for Therapeutic Radiology and Oncology
2010;28(3):147-154
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: To investigate the radiosensitizing effect of the selective epidermal growth factor receptor (EGFR) inhibitor nimotuzumab in human colorectal cancer cell lines. MATERIALS AND METHODS: Four human colorectal cancer cell lines, HCT-8, LoVo, WiDr, and HCT-116 were treated with nimotuzumab and/or radiation. The effects on cell proliferation, viability, and cell cycle progression were measured by MTT, clonogenic survival assay, flow cytometry, and Western blot. RESULTS: An immunoblot analysis revealed that EGFR phosphorylation was inhibited by nimotuzumab in colorectal cancer cell lines. Under these experimental conditions, pre-treatment with nimotuzumab increased radiosensitivity of colorectal cancer cell lines, except for cell line HCT-116. However, cell proliferation or cell cycle progression was not affected by the addition of nimotuzumab, irrespective of irradiation. CONCLUSION: Nimotuzumab enhanced the radiosensitivity of colorectal cancer cells in vitro by inhibiting EGFR-mediated cell survival signaling. This study provided a rationale for the clinical application of the selective EGFR inhibitor, nimotuzumab in combination with radiation in colorectal cancer cells.
