Photodynamic Effects of Radachlorin(R) on Cervical Cancer Cells.
- Author:
Su Mi BAE
1
;
Yong Wook KIM
;
Joon Mo LEE
;
Sung Eun NAMKOONG
;
Sei Jun HAN
;
Jong Ki KIM
;
Chang Hee LEE
;
Heung Jae CHUN
;
Hyun Sun JIN
;
Woong Shick AHN
Author Information
1. Catholic Research Institutes of Medical Science, The Catholic University of Korea College of Medicine, Seoul, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
Radachlorin(R);
Photodynamic therapy (PDT);
Cervical cancer;
TC-1 cell
- MeSH:
Animals;
Cell Line, Tumor;
Mice;
Necrosis;
Photochemotherapy;
Uterine Cervical Neoplasms*
- From:Cancer Research and Treatment
2004;36(6):389-394
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Photodynamic therapy (PDT) is a novel treatment modality, which produces local tissue necrosis with laser light following the prior administration of a photosensitizing agent. Radachlorin(R) has recently been shown to be a promising PDT sensitizer. In order to elucidate the antitumor effects of PDT using Radachlorin(R) on cervical cancer, growth inhibition studies on a HPV-associated tumor cell line, TC-1 cells in vitro and animals with an established TC-1 tumor in vivo were determined. MATERIALS AND METHODS: TC-1 tumor cells were exposed to various concentrations of Radachlorin(R) and PDT, with irradiation of 12.5 or 25 J/cm2 at an irradiance of 20 mW/cm2 using a Won-PDT D662 laser at 662 nm in vitro. C57BL/6 mice with TC-1 tumor were injected with Radachlorin(R) via different routes and treated with PDTin vivo. A growth suppression study was then used to evaluate the effects at various time points after PDT. RESULTS: The results showed that irradiation of TC-1 tumor cells in the presence of Radachlorin(R) induced significant cell growth inhibition. Animals with established TC-1 tumors exhibited significantly smaller tumor sizes over time when treated with Radachlorin(R) and irradiation. CONCLUSION: PDT after the application of Radachlorin(R) appears to be effective against TC-1 tumors both in vitro and in vivo.
