Enhancement of Allergen-induced Airway Inflammation by NOX2 Deficiency.
- Author:
Hee Yeon WON
1
;
Eun Jung JANG
;
Hyun Jung MIN
;
Eun Sook HWANG
Author Information
- Publication Type:Original Article
- Keywords: NADPH oxidase 2; Ovalbumin; Airway inflammation; Interleukin-17; Interferon-gamma
- MeSH: Animals; Cell Proliferation; Cytokines; Eosinophils; Goblet Cells; Hyperplasia; Inflammation; Interferon-gamma; Interleukin-17; Lung; Lymphocytes; Macrophages; Mice; Mucus; NADPH Oxidase; Neutrophils; Ovalbumin; Pneumonia; Reactive Oxygen Species; T-Lymphocytes; T-Lymphocytes, Regulatory
- From:Immune Network 2011;11(3):169-174
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: NADPH oxidase (NOX) modulates cell proliferation, differentiation and immune response through generation of reactive oxygen species. Particularly, NOX2 is recently reported to be important for regulating Treg cell differentiation of CD4+ T cells. METHODS: We employed ovalbumin-induced airway inflammation in wild-type and NOX2-deficient mice and analyzed tissue histopathology and cytokine profiles. RESULTS: We investigated whether NOX2-deficiency affects T cell-mediated airway inflammation. Ovalbumin injection which activates T cell-mediated allergic response increased airway inflammation in wild-type mice, as evidenced by increased immune cell infiltration, allergic cytokine expression, and goblet cell hyperplasia in the lung. Interestingly, NOX2 knockout (KO) mice were more susceptible to allergen-induced lung inflammation compared to wild-type mice. Immune cells including neutrophils, lymphocytes, macrophages, and eosinophils were drastically infiltrated into the lung of NOX2 KO mice and mucus secretion was substantially increased in deficiency of NOX2. Furthermore, inflammatory allergic cytokines and eotaxin were significantly elevated in NOX2 KO mice, in accordance with enhanced generation of inflammatory cytokines interleukin-17 and interferon-gamma by CD4+ T cells. CONCLUSION: These results indicate that NOX2 deficiency favorably produces inflammatory cytokines by T cells and thus increases the susceptibility to severe airway inflammation.
