Bone Healing Properties of Autoclaved Autogenous Bone Grafts Incorporating Recombinant Human Bone Morphogenetic Protein-2 and Comparison of Two Delivery Systems in a Segmental Rabbit Radius Defect.
- Author:
Eun Joo CHOI
1
;
Sang Hoon KANG
;
Hyun Jin KWON
;
Sung Won CHO
;
Hyung Jun KIM
Author Information
1. Department of Oral and Maxillofacial Surgery, Yonsei University College of Dentistry, Korea. kimoms@yuhs.ac
- Publication Type:Original Article
- Keywords:
Bone morphogenetic protein 2;
Autogenous autoclaved bone;
Critical bone defect;
Rabbit radius;
BMP carrier
- MeSH:
Animals;
Bone Morphogenetic Protein 2;
Collagen;
Escherichia;
Fibrin Tissue Adhesive;
Humans;
Porifera;
Rabbits;
Radius*;
Transplants*
- From:Maxillofacial Plastic and Reconstructive Surgery
2014;36(3):94-102
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: This study aims to validate the effect of autoclaved autogenous bone (AAB), incorporating Escherichia coli-derived recombinant human bone morphogenetic protein-2 (ErhBMP-2), on critical-sized, segmental radius defects in rabbits. Delivery systems using absorbable collagen sponge (ACS) and fibrin glue (FG) were also evaluated. METHODS: Radius defects were made in 12 New Zealand white rabbits. After autoclaving, the resected bone was reinserted and fixed. The animals were classified into three groups: only AAB reinserted (group 1, control), and AAB and ErhBMP-2 inserted using an ACS (group 2) or FG (group 3) as a carrier. Animals were sacrificed six or 12 weeks after surgery. Specimens were evaluated using radiology and histology. RESULTS: Micro-computed tomography images showed the best bony union in group 2 at six and 12 weeks after operation. Quantitative analysis showed all indices except trabecular thickness were the highest in group 2 and the lowest in group 1 at twelve weeks. Histologic results showed the greatest bony union between AAB and radial bone at twelve weeks, indicating the highest degree of engraftment. CONCLUSION: ErhBMP-2 increases bony healing when applied on AAB graft sites. In addition, the ACS was reconfirmed as a useful delivery system for ErhBMP-2.
