Protective effects of pine bark extract against cisplatin-induced hepatotoxicity and oxidative stress in rats.
10.5625/lar.2014.30.4.174
- Author:
Je Won KO
1
;
In Chul LEE
;
Sung Hyuk PARK
;
Changjong MOON
;
Seong Soo KANG
;
Sung Ho KIM
;
Jong Choon KIM
Author Information
1. College of Veterinary Medicine, Chonnam National University, Gwangju, Korea. toxkim@jnu.ac.kr
- Publication Type:Original Article
- Keywords:
Cisplatin;
hepatotoxicity;
oxidative stress;
pine bark extract;
protective effects
- MeSH:
Animals;
Catalase;
Cisplatin;
Glutathione;
Glutathione Transferase;
Hepatocytes;
Humans;
Injections, Intraperitoneal;
Lipid Peroxidation;
Liver;
Male;
Malondialdehyde;
Oxidative Stress*;
Rats*;
Superoxide Dismutase
- From:Laboratory Animal Research
2014;30(4):174-180
- CountryRepublic of Korea
- Language:English
-
Abstract:
We investigated the protective effects of pine bark extract (pycnogenol(R), PYC) against cisplatin-induced hepatotoxicity and oxidative stress in rats. Twenty-four male rats were divided into the following four groups: (1) vehicle control, (2) cisplatin (7.5 mg/kg), (3) cisplatin & PYC 10 (10 mg/kg/day), and (4) cisplatin & PYC 20 (20 mg/kg/day). A single intraperitoneal injection of cisplatin induced hepatotoxicity, as evidenced by an increase in serum aminotransferase and histopathological alterations, including degeneration/necrosis of hepatocytes, vacuolation, and sinusoidal dilation. In addition, an increase in the malondialdehyde (MDA) concentration and a decrease in the reduced glutathione (GSH) content and catalase (CAT), superoxide dismutase (SOD), and glutathione S-transferase (GST) activities were observed in the cisplatin-treated rat hepatic tissues. In contrast, PYC treatment effectively prevented cisplatin-induced hepatotoxicity, including the elevation of aminotransferase and histopathological lesions, in a dosedependent manner. Moreover, PYC treatment also induced antioxidant activity by decreasing MDA level and increasing GSH content and SOD and GST activities in liver tissues. These results indicate that PYC has a protective effect against acute hepatotoxicity induced by cisplatin in rats, and that the protective effects of PYC may be due to inhibiting lipid peroxidation and increasing antioxidant activity.
