Unilateral cryptorchidism induces morphological changes of testes and hyperplasia of Sertoli cells in a dog.
10.5625/lar.2014.30.4.185
- Author:
Joon Ho MOON
1
;
Dae Young YOO
;
Young Kwang JO
;
Geon A KIM
;
Hyo Young JUNG
;
Jung Hoon CHOI
;
In Koo HWANG
;
Goo JANG
Author Information
1. Laboratory of Theriogenology and Biotechnology, Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Seoul National University, Seoul, Korea. snujang@snu.ac.kr
- Publication Type:Case Report
- Keywords:
Dog;
efferent ductule;
seminiferous tubule;
sertoli cells;
unilateral cryptorchidism
- MeSH:
Animals;
Causality;
Cell Proliferation;
Cryptorchidism*;
Dogs*;
Eosine Yellowish-(YS);
Epididymis;
Hematoxylin;
Hyperplasia*;
Immunohistochemistry;
Male;
Orchiectomy;
Seminiferous Tubules;
Sertoli Cells*;
Spermatids;
Spermatocytes;
Spermatogonia;
Testis*
- From:Laboratory Animal Research
2014;30(4):185-189
- CountryRepublic of Korea
- Language:English
-
Abstract:
Cryptorchidism is one of the most common genital defects in dogs. This study investigated the effects of abdominal cryptorchidism on morphology, cell proliferation, and Sertoli cell condition in a dog with spontaneous unilateral cryptorchidism. Elective orchidectomy was performed on the abdominal right testis and the scrotal left testis. Significant reductions in numbers of spermatogonia, spermatocytes, and spermatids were observed in hematoxylin and eosin stained sections of the cryptorchid testis. The size of the epididymal duct was smaller than that of the control testis. Based on Ki67 immunohistochemistry, the proliferative activity of spermatogonia and spermatocytes was significantly decreased in the cryptorchid testis. However, proliferative activity was increased in the epididymal duct. Based on GATA-4 immunohistochemistry, Sertoli cells were relatively resistant to cryptorchidism, and the proliferative activity of Sertoli cells was markedly increased in the cryptorchid testis than in the control testis. These results suggest that spontaneous unilateral cryptorchidism causes morphological defects in spermatogonia and spermatocytes in the testis and changes the size of the efferent ductule of the epididymis. In addition, spontaneous unilateral cryptorchidism increases proliferative activity of Sertoli cells, which may be a predisposing factor for Sertoli cell cancer in cryptorchid testes.
