Expression of Glucagon-Like Peptide 1 Receptor during Osteogenic Differentiation of Adipose-Derived Stem Cells.
10.3803/EnM.2014.29.4.567
- Author:
Yun Kyung JEON
1
;
Min Jung BAE
;
Ju In KIM
;
Joo Hyoung KIM
;
Soo Jong CHOI
;
Su Kyoung KWON
;
Joon Hyop AN
;
Sang Soo KIM
;
Bo Hyun KIM
;
Yong Ki KIM
;
In Joo KIM
Author Information
1. Department of Internal Medicine, Pusan National University School of Medicine, Korea. injkim@pusan.ac.kr
- Publication Type:Original Article
- Keywords:
Glucagon-like peptide 1;
Glucagon-like peptide 1 receptor;
Adipose-derived stem cell;
Osteogenesis
- MeSH:
Actins;
Alkaline Phosphatase;
Anabolic Agents;
Bone and Bones;
Cell Line;
Gene Expression;
Glucagon-Like Peptide 1*;
Humans;
Incretins;
Male;
Osteoblasts;
Osteocalcin;
Osteogenesis;
Real-Time Polymerase Chain Reaction;
RNA, Messenger;
Stem Cells*;
Subcutaneous Fat;
Surgery, Plastic;
Tissue Donors;
Up-Regulation;
Glucagon-Like Peptide-1 Receptor
- From:Endocrinology and Metabolism
2014;29(4):567-573
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Glucagon-like peptide 1 (GLP-1), an incretin hormone well known for its glucose-lowering effect, was recently reported to exert an anabolic effect on bone. Although the exact mechanism is not known, it likely involves the GLP-1 receptor (GLP-1R), which is expressed in some osteoblastic cell lines. Adipose-derived stem cells (ADSCs) have mesenchymal stem cell-specific characteristics, including osteoblastic differentiation potential. We evaluated the expression of GLP-1R during osteogenic differentiation of ADSCs. METHODS: ADSCs were isolated from subcutaneous adipose tissue obtained from three male donors during plastic surgery and were subjected to osteogenic induction. Mineralization was assessed by Alizarin Red staining on day 21. Expression of alkaline phosphatase (ALP), osteocalcin (OC), and GLP-1R was measured by real-time polymerase chain reaction in triplicate for each patient on days 0, 7, 14, and 21. Target mRNA expression levels were normalized to that of beta-actin. RESULTS: ADSCs were fibroblast-like in morphology, adhered to plastic, and had multipotent differentiation potential, as assessed using specific antigen markers. The osteogenic markers ALP and OC were notably upregulated at 21 days. Osteogenic differentiation resulted in a time-dependent increase in the expression of GLP-1R (P=0.013). CONCLUSION: We demonstrated upregulation of GLP-1R gene expression during osteogenic differentiation of ADSCs. This finding suggests that GLP-1 may induce osteogenic differentiation in bone tissue.
