The Effect of Human Placental Extract on Rheumatoid Arthritis in an Animal Model.
10.5535/arm.2012.36.2.197
- Author:
Jeong Dong PARK
1
;
Sang Il LEE
;
A Ram KIM
;
Jong Moon PARK
;
Sang Yeop SHIN
;
Jun Hwa SHIN
;
Seung Won MOON
;
Hyun PARK
;
Min Kyun OH
;
Hee Suk SHIN
Author Information
1. Department of Rehabilitation Medicine, Gyeongsang National University College of Medicine, Jinju 660-702, Korea. hsshin@nongae.gsnu.ac.kr
- Publication Type:Original Article
- Keywords:
Human placental extract (HPE);
Rheumatoid arthritis (RA)
- MeSH:
Animals;
Arthritis;
Arthritis, Experimental;
Arthritis, Rheumatoid;
Cartilage;
Cytokines;
Enzyme-Linked Immunosorbent Assay;
Humans;
Incidence;
Inflammation;
Interleukin-10;
Interleukin-6;
Joints;
Mice;
Mice, Inbred NOD;
Models, Animal;
Osteoclasts;
Tumor Necrosis Factor-alpha
- From:Annals of Rehabilitation Medicine
2012;36(2):197-206
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: To assess the efficacy of human placental extract (HPE) in an animal model of rheumatoid arthritis (RA). METHOD: We used (i) KRN C57BL/6 TCR transgenic x NOD mice (KBx/N) serum transfer arthritis and (ii) collagen-induced arthritis (CIA) mice to evaluate the effi cacy of HPE (1 ul or 100 ul, intra-peritoneal, three times per week) on RA. Incidence, severity of arthritis, and hind-paw thickness were quantifi ed. Joint destruction was analyzed using modifi ed mammographic imaging. Histopathological analysis for inflammation, cartilage, and osteoclasts was performed using Hematoxylin-eosin (H-E), safranin-O, and tartrate-resistant acidic phosphatase (TRAP). ELISAs were used for detection of various cytokines in serum and joint tissue. RESULTS: There were no significant differences in incidence of arthritis, clinical scores of arthritis, and hind-paw thickness between HPE-treated and vehicle-treated groups for up to 2 weeks in the KBx/N serum transfer arthritis model. Histopathological analysis also showed no differences 2 weeks after treatment. Levels of TNF-alpha, IL-1beta, IL-6, IL-10, and RANKL in serum and joint tissues were similar in all groups. Furthermore, there were no differences in clinical, radiological, and histological parameters between HPE-treated and vehicle-treated group for 3 weeks in the CIA model. CONCLUSION: Systemic treatment with HPE has no beneficial effects on arthritis in animal models of RA. Therefore, indiscreet use of HPE in RA should be forbidden.
