Usage of T Cell receptor repertoire is restricted in synovial lymphocytes in rheumatoid arthritis.
- Author:
Dae Ho KWON
1
;
Soo Kon LEE
;
Se Jong KIM
;
In Hong CHOI
Author Information
- Publication Type:Original Article
- Keywords: rheumatoid arthritis; synovial membrane; TCR beta - Chain
- MeSH: Antibodies, Monoclonal; Arthritis, Rheumatoid*; Autoimmune Diseases; Humans; Joints; Lymphocytes*; Polymerase Chain Reaction; Receptors, Antigen, T-Cell*; Synovial Membrane; T-Lymphocytes
- From:Immune Network 2001;1(1):70-76
- CountryRepublic of Korea
- Language:Korean
- Abstract: BACKGROUND: Rheumatoid arthritis is an autoimmune disease characterized by a chronic inflammatory process, primarily involving the synovial membrane of peripheral joints, where T cell activation is found. To address the superantigen stimulation in rheumatoid arthritis, T cell clonality and the expression of activation markers were analyzed. METHODS: To detect TCRB V usage, inverse PCR and sequencing were done. Monoclonal antibodies were used for flow cytometric analysis of TCRBV8 or TCRBV5. As result s, a restricted usage of TCRBV3 gene was detected in synovial lymphocytes from one rheumatoid arthritis patient . However, preferential usage for TCRB V8, which may be one indicator for stimulation by staphylococcal superantigen, was not obvious although general activation of T cells was found as high DR+ percentage in synovial T cells. These data show specific antigen rather than superantigen might involve the pathogenesis of rheumatoid arthritis.
