Effects of the Luteolin-gadolinium complex on C48/80-induced inflammatory responses in HMC-1 mast cells
- VernacularTitle:Лютеолин-гадолиниум нэгдлийн C48/80-өөр өдөөгдсөн HMC-1 шигүү мөхлөгт эсийн үрэвслийн урвалд үзүүлэх нөлөөг судалсан дүн
- Author:
Surui A
1
;
2
;
Enkhtur Y
1
;
Dugarmaa U
1
Author Information
1. Department of Dermatology, School of Medicine, MNUMS
2. Department of Dermatology, The First Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology
- Publication Type:Journal Article
- Keywords:
luteolin-gadolinium;
urticaria;
mast cells;
anti-inflammatory activity
- From:
Mongolian Journal of Health Sciences
2026;97(7):83-87
- CountryMongolia
- Language:Mongolian
-
Abstract:
Background:Mast cell activation and the release of inflammatory mediators play central roles in the pathogenesis of urticaria. Luteolin possesses anti-inflammatory and anti-allergic properties, whereas the luteolin-gadolinium complex (Lut-Gd) has demonstrated anti-inflammatory activity in macrophage models. However, the effects of Lut-Gd on mast cell activation remain unclear.
Aim:To evaluate the effects of Lut-Gd on cell viability, cytotoxicity, and inflammatory mediator release in Compound 48/80 (C48/80)-stimulated HMC-1 cells.
Materials and Methods:HMC-1 cells were stimulated with C48/80 and treated with either luteolin or Lut-Gd. Cell viability and membrane integrity were assessed using CCK-8 and lactate dehydrogenase (LDH) release assays, respectively. Histamine, IL-1β, IL-6, and TNF-α concentrations in culture supernatants were quantified by enzyme-linked immunosorbent assay (ELISA).
Results:At the concentrations selected for subsequent experiments, C48/80 (10 μg/mL), luteolin (2.5 μg/mL), and Lut-Gd (10 μg/mL) did not induce significant cytotoxicity. C48/80 stimulation significantly increased the release of histamine, IL-1β, IL-6, and TNF-α from HMC-1 cells. Treatment with either luteolin or Lut-Gd significantly reduced the levels of these inflammatory mediators (p < 0.05).
Conclusion:Lut-Gd suppressed C48/80-induced release of histamine and inflammatory cytokines from HMC-1 cells without inducing significant cytotoxicity, supporting its potential anti-inflammatory activity in mast cell-mediated inflammation.
- Full text:202611022149283589614. Лютеолин-гадолиниум нэгдлийн C48-80-өөр өдөөгдсөн HMC-1 шигүү мөхлөгт эсийн үрэвслийн.pdf
