Protective effect of Indigo Naturalis on cerebral ischemia-reperfusion injury in mice
10.12206/j.issn.2097-2024.202510044
- VernacularTitle:青黛对脑缺血再灌注损伤小鼠的保护作用研究
- Author:
Yuchen GUO
1
;
Junchi LI
1
;
Guoling YANG
2
;
Yuefan ZHANG
2
;
Tiejun LI
1
;
Lili SUN
1
Author Information
1. Shanghai Punan Hospital of Pudong New District, Shanghai 200125, China.
2. Shanghai University, Shanghai 200444, China.
- Publication Type:Originalarticles
- Keywords:
Indigo Naturalis;
cerebral ischemia-reperfusion injury;
neurological function scores;
inflammatory response
- From:
Journal of Pharmaceutical Practice and Service
2026;44(10):508-514
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of Indigo Naturalis on cerebral ischemia-reperfusion injury in mice. Methods The mouse model of middle cerebral artery occlusion/reperfusion (MCAO/R) was established using the filament ligation method. Mice were randomly divided into five groups: sham surgery, model, positive drug control, low-dose Indigo Naturalis, and high-dose Indigo Naturalis. After 7 d of drug administration, the relevant experiments were performed. Neurological function was assessed via behavioral scoring, balance beam test, forelimb grip strength, and tail lift test. Brain infarct volume was quantified using 2,3,5-triphenyl tetrazolium chloride (TTC) staining. Histopathological changes and neuronal apoptosis were systematically analyzed through hematoxylin-eosin (HE) staining, Nissl staining, and TUNEL staining. Finally, polymerase chain reaction (PCR) was employed to detect mRNA expression levels of inflammatory cytokines interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and microglial polarization markers (CD32, CD206) in the mouse cerebral cortex. Results Compared with the model group, the neurological function scores of mice in the high-dose Indigo Naturalis group showed significant improvement, and the motor ability recovery in each drug-administration group was remarkable (P<0.05, P<0.01). The cerebral infarct volume in the high-dose Indigo Naturalis group was significantly reduced (P<0.05). Histological results revealed that Indigo Naturalis could alleviate pathological damage in brain tissue and reduce neuronal apoptosis (P<0.01). PCR results demonstrated that after treatment with Indigo Naturalis, the expression of inflammatory factors and CD32 was significantly downregulated (P<0.05, P<0.01), while the expression of CD206 was significantly upregulated (P<0.05). Conclusion Indigo Naturalis could improve motor function in mice with cerebral ischemia-reperfusion injury, and its effects might be related to regulating inflammatory responses and reducing neuronal damage.
