Comparison of the effects of five Blastocystis sp. staining methods
10.3969/j.issn.1005-0507.2026.03.001
- VernacularTitle:芽囊原虫5种不同染色方法效果比较
- Author:
Ao-kun ZHANG
1
;
Long-fei CHEN
2
;
Jing LI
1
;
Hui-zhen YUAN
1
;
Sen-yang LI
1
;
Fu-chun JIAN
1
Author Information
1. College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, Henan, China
2. Yongcheng Animal Husbandry Development Service Center, Yongcheng 476600, Henan, China
- Publication Type:Journal Article
- Keywords:
Blastocystis;
Morphological observation;
Wright-Giemsa staining;
Periodic acid schiff staining;
Oil red O staining;
Nile red staining;
Lugo's iodine solution staining
- From:
Acta Parasitologica et Medica Entomologica Sinica
2026;33(3):161-167,172
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the efficacy of five Blastocystis staining method for staining cells with different morphologies and in different developmental stages to select appropriate staining techniques for specific scenarios and experimental purposes. Methods Blastocystis was stained using Wright-Giemsa, periodic acid-Schiff (PAS), Oil Red O, Nile Red, and Lugol's iodine solution respectively. The staining effects of the five method on Blastocystis were compared. Results Wright-Giemsa staining colored the cytoplasm and nucleus of Blastocystis bluish-purple, with other parts lightly stained; PAS staining rendered Blastocystis cells red; Oil Red O staining showed different structures within Blastocystis in varying intensities of orange-red; Nile Red enabled Blastocystis to fluoresce orange-yellow under a fluorescence microscope; Lugol's iodine solution stained the cytoplasm and nucleus a dark brownish-yellow. Conclusions Wright-Giemsa staining is recommended for clinical diagnosis. For metabolic studies, a combination of periodic acid-Schiff (PAS) staining and Oil Red O staining is suggested; PAS + Oil Red O staining can provide comprehensive morphological evidence for cell metabolism research. Nile Red staining is recommended for dynamic observation of live Blastocystis. Lugol's iodine staining is suitable for preliminary screening of large-scale samples.
- Full text:2026102023463923353COMPARISON OF THE EFFECTS OF FIVE BLASTOCYSTIS SP. STAINING METHODS_.pdf
