Predictive screening，activity evaluation and quantitative analysis of hepatoprotective constituents from Thesium chinense Turcz.
- VernacularTitle:百蕊草保肝成分的预测筛选、活性评价及定量分析
- Author:
Li LI
1
;
Xuechun CHENG
2
;
Xiuping ZHUANG
2
;
Peng ZHOU
1
;
Baoqing ZHANG
1
;
Xin WANG
3
Author Information
1. First Clinical Medical College，Shandong University of Traditional Chinese Medicine，Jinan 250355，China;Dept. of Pediatrics，Affiliated Hospital of Shandong University of Traditional Chinese Medicine，Jinan 250011，China
2. School of Pharmacy，Shandong University of Traditional Chinese Medicine，Jinan 250355，China
3. Institute of Traditional Chinese Medicine Innovation and Transformation，Affiliated Hospital of Shandong University of Traditional Chinese Medicine，Jinan 250011，China
- Publication Type:Journal Article
- Keywords:
Thesium chinense Turcz.;
hepatoprotective active constituents;
Network pharmacology;
Zebrafish;
kaempferol-3- O -glucorhamnoside;
buddleoside;
kaempferol-3，7-di- O -glucoside;
apigenin-5- O -neohesperidoside
- From:
China Pharmacy
2026;37(19):2522-2528
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To provide an experimental basis for quality control， clinical application and development of hepatoprotective drugs derived from Thesium chinense Turcz.METHODS Network pharmacology was adopted to predict and screen potential hepatoprotective constituents and their action targets of T. chinense Turcz. A liver-injured model of transgenic zebrafish Tg （ fabp10a ： dsRed ） with fluorescent-labeled liver was induced by 4 mmol/L isoniazid. The relative liver fluorescence area and relative fluorescence intensity of zebrafish larvae， as well as the levels of aspartate aminotransferase （AST）， alanine aminotransferase （ALT）， glutathione （GSH） and interleukin-6 （IL-6） were taken as indexes to evaluate the hepatoprotective activity of potential active constituents. High-performance liquid chromatography was used to determine the contents of hepatoprotective active constituents in 17 batches of T. chinense Turcz. medicinal materials from different producing areas.RESULTS Four potential hepatoprotective constituents including kaempferol-3- O -glucorhamnoside （KOG）， kaempferol-3，7-di- O -glucoside （KDG）， buddleoside （BUD） and apigenin-5- O -neohesperidoside （AON） were screened out via network pharmacology， and their core targets involved mitogen-activated protein kinase 1， nuclear factor-κB subunit 1， etc. Pharmacodynamic verification results showed that 25 μmol/L KOG， BUD， AON and 50 μmol/L KDG could significantly increase the relative liver fluorescence area and relative fluorescence intensity of liver-injured model larvae （ P ＜0.05）， reduce the levels of AST， ALT and IL-6 （ P ＜0.05）， and elevate the GSH level （ P ＜0.05）. Content determination results indicated that KOG could be detected in all 17 batches of T. chinense Turcz. medicinal materials， with contents ranging from 0.097 0 to 8.082 0 mg/g；KDG， AON and BUD were not detected in partial batches of samples， and their contents in detectable batches were 0.019 3-0.123 2 mg/g， 0.018 4-0.109 1 mg/g and 0.385 1-1.189 3 mg/g， respectively.CONCLUSIONS KOG， KDG， AON and BUD in T.chinense Turcz. may be its potential hepatoprotective active constituents. Among them， KOG can be detected in T.chinense Turcz. medicinal materials from different producing areas with relatively high content， which can be used as the main marker constituent for evaluating the hepatoprotective effect of T.chinense Turcz.
