Paeoniflorin Ameliorates Neuroinflammation and Cognitive Impairment in Rat Model of Alzheimer's Disease via JAK2/STAT3 Signaling Pathway
10.13422/j.cnki.syfjx.20260406
- VernacularTitle:基于JAK2/STAT3信号通路探讨芍药苷改善阿尔茨海默病大鼠神经炎症及认知障碍的作用机制
- Author:
Mengfen ZHOU
1
;
Shuyao TANG
1
;
Xin SUN
1
;
Gangying FU
1
;
Xinxing ZHOU
1
;
Jiawei HE
1
;
Wenjing YU
1
;
Ping LI
1
;
Shaowu CHENG
1
;
Zhenyan SONG
1
Author Information
1. Hunan Province Key Laboratory of Cerebrovascular Disease Prevention and Treatment of Integrated Traditional Chinese and Western Medicine， School of Integrated Chinese and Western Medicine， Hunan University of Chinese Medicine，Changsha 410208，China
- Publication Type:Journal Article
- Keywords:
paeoniflorin;
Alzheimer's disease;
neuroinflammation;
Janus kinase 2 （JAK2）/signal transducer and activator of transcription 3 （STAT3） signaling pathway;
cognitive impairment
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2026;32(22):138-147
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effects of paeoniflorin （PF） on neuroinflammation and cognitive impairment in the rat model of Alzheimer's disease （AD） and to clarify whether its mechanism is associated with regulation of the Janus kinase 2 （JAK2）/signal transducer and activator of transcription 3 （STAT3） signaling pathway. MethodsGlycosides related to AD-associated neuroinflammation were screened through bibliometric analysis， and the potential targets of PF were predicted through network pharmacology and molecular docking. A rat model of AD was established by intraperitoneal injection of D-galactose （100 mg·kg-1·d-1）. Rats were assigned into a control group， a model group， a PF （200 mg·kg-1） group， and a donepezil （0.5 mg·kg-1·d-1） group， and drugs were administered by gavage for 21 days. The learning and memory abilities were evaluated by the Morris water maze （MWM）. Hematoxylin-eosin （HE） staining and Nissl staining were performed to observe hippocampal neuronal morphology. The mRNA levels of inflammatory factors were determined by Real-time quantitative polymerase chain reaction （Real-time PCR）， and the protein levels of molecules in the JAK2/STAT3 signaling pathway were determined by Western blot. ResultsCompared with the control group， the model group showed prolonged escape latency and decreased platform crossings and time spent in the target quadrant （P<0.05， P<0.01）， accompanied by increased expression of interleukin-1β （IL-1β） and tumor necrosis factor-α （TNF-α）， as well as elevated ratios of phosphorylated （p）-JAK2/JAK2 and p-STAT3/STAT3 （P<0.01）. Compared with the model group， the PF group and donepezil group exhibited shortened escape latency and increased platform crossings and time spent in the target quadrant （P<0.05， P<0.01）， decreased expression levels of IL-1β and TNF-α， upregulated mRNA levels of interleukin-4 （IL-4） and interleukin-10 （IL-10）， and reduced ratios of p-JAK2/JAK2 and p-STAT3/STAT3 （P<0.05， P<0.01）. ConclusionPaeoniflorin can alleviate cognitive impairment and neuroinflammation in the rat model of AD by inhibiting the JAK2/STAT3 signaling pathway.
