The effects of lncRNA CYTOR on the proliferation, invasion, and epithelial-mesenchymal transition of endometrial cancer HEC-1-A cells by targeting miR-488-3p/PUM2
DOI：10.3872/j.issn.1007-385X.2026.09.002
- VernacularTitle:lncRNA CYTOR通过miR-488-3p/PUM2通路影响子宫内膜癌HEC-1-A细胞增殖、侵袭及上皮-间充质转化
- Author:
Zhu Yufen1
1
,
2
;
Xu Leichan2
1
,
2
;
Bi Kehao1
1
,
2
;
Du Shipu1
1
,
2
Author Information
1. Department of Pathology, Fourth Hospital of Shijiazhuang City, Shijiazhuang 050011, Hebei, China;
2. Department of Gynecology, Fourth Hospital of Shijiazhuang City, Shijiazhuang 050011, Hebei, China
- Publication Type:Journal Article
- Keywords:
lncRNA CYTOR；miR-488-3p/PUM2；子宫内膜癌；增殖；上皮-间充质转化；侵袭
- From:
Chinese Journal of Cancer Biotherapy
2026;33(9):943-950
- CountryChina
- Language:Chinese
-
Abstract:
[摘 要] 目的：探讨lncRNA CYTOR靶向miR-488-3p/Pumilio RNA结合家族成员2（PUM2）对子宫内膜癌（EC）细胞增殖、侵袭及上皮-间充质转化（EMT）的影响。方法：RT-qPCR检测EC细胞及hEM15A细胞中的基因表达情况；将HEC-1-A细胞随机分为空白组、sh-NC组、sh-lncRNA CYTOR组、mimic NC组、miR-488-3p mimic组、sh-lncRNA CYTOR + inhibitor NC组、sh-lncRNA CYTOR + miR-488-3p inhibitor组、miR-488-3p mimic + vector组、miR-488-3p mimic + PUM2组，检测各组HEC-1-A细胞增殖、侵袭、基因表达及EMT相关蛋白E-cadherin、N-cadherin、增殖蛋白（Ki-67）表达；双萤光素酶及RNA免疫沉淀（RIP）实验验证miR-488-3p与lncRNA CYTOR、PUM2的靶向关系。结果：EC细胞中miR-488-3p表达降低，lncRNA CYTOR、PUM2 mRNA表达增加（P < 0.05），以HEC-1-A细胞中的变化最为明显；沉默lncRNA CYTOR可降低细胞增殖、侵袭能力及Ki-67、N-cadherin、lncRNA CYTOR、PUM2表达水平，上调E-cadherin、miR-488-3p表达（P < 0.05）；过表达miR-488-3p可降低细胞增殖、侵袭能力及Ki-67、N-cadherin、PUM2表达水平，增加E-cadherin、miR-488-3p表达（P < 0.05）；抑制miR-488-3p逆转沉默lncRNA CYTOR对细胞增殖、侵袭、EMT的抑制作用；过表达PUM2逆转过表达miR-488-3p对细胞增殖、侵袭、EMT的抑制作用；miR-488-3p与lncRNA CYTOR、PUM2具有靶向关系。结论：沉默lncRNA CYTOR通过调控miR-488-3p/PUM2抑制EC细胞增殖、侵袭和EMT。
- Full text:2026101615542805191202609002.pdf
