Acan-Gal-1 Modulates Macrophage Immune Phenotypes through Membrane Annexin A2
10.11714/jsysu.med.YX20250132
- VernacularTitle:Acan-Gal-1通过膜Annexin A2调控巨噬细胞免疫表型
- Author:
Lisha XIE
1
;
Jiayang WANG
1
;
Baolong YAN
1
;
Wei ZHAO
1
;
Huicong HUANG
1
Author Information
1. School of Basic Medical Sciences， Wenzhou Medical University， wenzhou 325400， China
- Publication Type:Journal Article
- Keywords:
Acan-Gal-1;
macrophage polarization;
annexin A2;
S100 calcium binding protein A10;
classically activated macrophage;
alternatively activated macrophage;
Angiostrongylus cantonensis
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2026;47(1):17-28
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the molecular mechanisms by which Acan-Gal-1 regulates macrophage immune phenotypes and Annexin A2/S100A10 membrane translocation in Angiostrongylus cantonensis. MethodsImmunofluorescence assay （IFA） was performed to determine the subcellular localization of Annexin A2 and S100A10 in macrophages. Flow cytometry and ELISA were used to assess functional changes in macrophage. siRNA interference of Annexin A2 was used to detect the effect of Acan-Gal-1 on various indicators. The expression levels of related genes and proteins were analyzed by Western blot， qRT-PCR， and ELISA methods. Bioinformatics analyses were conducted to characterize the structural features of Acan-Gal-1， and sequence alignment and molecular interaction prediction were performed to explore its potential interaction with Annexin A2. ResultsThis study found that Acan-Gal-1 can reverse LPS-induced promotion of macrophage migration and phagocytic function； it significantly upregulated M2-type markers in macrophages （Arg-1， IL-10， TGF-β） while inhibiting the expression of M1-type markers （iNOS， IL-6， IL-1β） induced by LPS. Acan-Gal-1 reduced the protein levels of Annexin A2 in macrophages under an immunologically activated state； after interfering with Annexin A2 expression， Acan-Gal-1 not only fails to induce macrophages to transition to an immunologically inhibitory phenotype but also fails to inhibit LPS-induced transition of macrophages to an immunologically activated phenotype. Although Acan-Gal-1 did not affect the expression of Annexin A2 or its chaperone protein S100A10 in macrophages， it markedly promoted their translocation to the plasma membrane. ConclusionsAcan-Gal-1 facilitates the enrichment of the Annexin A2/S100A10 complex at the macrophage membrane， thereby exerting immunomodulatory effects on macrophage polarization.
