Identification of Angiostrongylus cantonensis in Wild Rodent Feces via cytb Sequences
10.11714/jsysu.med.YX20250099
- VernacularTitle:应用cytb序列鉴定野鼠粪便中的广州管圆线虫感染
- Author:
Jinwei LÜ
1
;
Yu LIN
1
;
Yaoyu FENG
1
;
Lihua XIAO
1
;
Dongjuan YUAN
1
Author Information
1. College of Veterinary Medicine， South China Agricultural University， Guangzhou 510642， China
- Publication Type:Journal Article
- Keywords:
Angiostrongylus cantonensis;
cytb;
wild rodent;
feces;
investigation
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2026;47(1):11-16
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveSeveral molecular markers were evaluated for their reliability in detecting Angiostrongylus cantonensis in wild rodent feces and for determining the infection status of these rodents. MethodsA total of 128 wild rodents were captured in Huashan Town， Huadu district， Guangzhou. Their fecal samples were collected and total DNA was extracted with the FastDNA SPIN Kit for Soil. PCR amplification was performed to obtain cytb， cox I and ITS2 gene fragments， followed by Sanger sequencing and phylogenetical analysis. ResultsAmong all 128 fecal samples， only 5 had clean PCR bands and unambiguous cytb sequences suitable for reliable species identification. The infection rate of A. cantonensis was 7.58% （5/66） in Rattus norvegicus， whereas no cytb fragments were detected in Rattus tanezumi， Rattus losea， Suncus murinus and Bandicota indica. These data indicate that R. norvegicus is a highly suitable host for A. cantonensis， and the cytb gene serves as an effective molecular marker for detecting A. cantonensis in wild rodent feces. ConclusionThe cytb is an effective molecular marker for detecting A. cantonensis in wild rodent feces， enabling long-term surveillance of its prevalence and potential public health threat.
